Linthicum D S, Sell S
Immunology. 1977 May;32(5):701-8.
Equal numbers of rabbit peripheral blood lymphocytes (PBLs) label for either a- or b-group immunoglobulin allotypes using a modification of the mixed antiglobulin reaction of Coombs adapted for electron microscopy. This technique which employs immunoferritin markers is consistently more sensitive than other methods used for labelling surface Ig on lymphocytes. Although approximately 75 per cent of PBLs label for either a- or b-group allotypes, the number of immunoferritin grains on the cell surface after labelling for b group allotypes is nearly twice as great as that observed after labelling for a group allotypes. We conclude that essentially all Ig-bearing blood lymphocytes of the rabbit express both heavy chains (a allotypes) and light chains (b allotypes) on their surface membranes, but that light chain allotypic determinants are more accessible or exposed than the heavy chain (Fd) allotypic determinants.
使用一种适用于电子显微镜的改良库姆斯混合抗球蛋白反应，等量的兔外周血淋巴细胞（PBL）分别标记为a组或b组免疫球蛋白同种异型。这种采用免疫铁蛋白标记物的技术始终比用于标记淋巴细胞表面免疫球蛋白的其他方法更敏感。尽管约75%的PBL标记为a组或b组同种异型，但标记b组同种异型后细胞表面的免疫铁蛋白颗粒数量几乎是标记a组同种异型后观察到的数量的两倍。我们得出结论，兔的基本上所有携带免疫球蛋白的血液淋巴细胞在其表面膜上都表达重链（a同种异型）和轻链（b同种异型），但轻链同种异型决定簇比重链（Fd）同种异型决定簇更容易接近或暴露。
