Burka J F, Ali M, McDonald J W, Paterson N A
Prostaglandins. 1981 Nov;22(5):683-91. doi: 10.1016/0090-6980(81)90207-0.
Specific radioimmunoassays were used to demonstrate the synthesis by the guinea pig trachea of 6-keto PGF1 alpha, TxB2, and PGF2 alpha in addition to PGE2. The rank order of both spontaneous and stimulated release was PGE2 greater than PGF2 alpha greater than 6-keto PGF1 alpha = TxB2. Ovalbumin-induced prostanoid release from sensitized tissue was antigen-specific. The release was unlikely to be secondary consequence of tracheal contraction since incubations with calcium ionophore A23187, at a concentration which produces an equivalent magnitude of contraction of sensitized trachea, did not induce a significant PG or Tx production. In contrast, significantly higher prostanoid synthesis was induced by A23187 in unsensitized than sensitized trachea. Thus sensitization altered the profile of arachidonic acid metabolism evoked by the ionophore.
采用特异性放射免疫分析法证明，豚鼠气管除了能合成前列腺素E2（PGE2）外，还能合成6-酮前列腺素F1α（6-keto PGF1α）、血栓素B2（TxB2）和前列腺素F2α（PGF2α）。自发释放和刺激释放的顺序均为：PGE2＞PGF2α＞6-keto PGF1α = TxB2。卵清蛋白诱导致敏组织释放类前列腺素具有抗原特异性。这种释放不太可能是气管收缩的继发结果，因为用钙离子载体A23187孵育时，虽然该浓度能使致敏气管产生同等程度的收缩，但并未诱导显著的PG或Tx生成。相反，A23187在未致敏气管中诱导的类前列腺素合成明显高于致敏气管。因此，致敏改变了离子载体诱发的花生四烯酸代谢概况。
