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关于人凝胶过滤血小板中磷脂定量分析的重新思考。

Reconsideration of quantitative phospholipid analysis in human gel-filtered blood platelets.

作者信息

Ostermann G, Spangenberg P, Raithel C, Till U

出版信息

Acta Haematol. 1982;67(1):13-9. doi: 10.1159/000207019.

DOI:10.1159/000207019
PMID:6800196
Abstract

In the literature the values for phospholipid content and the pattern of human gel-filtered platelets diverge considerably. In order to find the possible reasons for these discrepancies, the separation of plasma constituents from gel-filtered platelets and the influence of different extraction methods were reinvestigated. The critical point in phospholipid determination of platelets appeared to be the completeness of plasma lipid separation from platelets and not the extraction method. Avoiding contamination by plasma lipids the results with gel-filtered platelets correlated to that obtained with washed platelets.

摘要

在文献中,人凝胶过滤血小板的磷脂含量值和模式差异很大。为了找出这些差异的可能原因,重新研究了从凝胶过滤血小板中分离血浆成分以及不同提取方法的影响。血小板磷脂测定的关键点似乎是血浆脂质与血小板分离的完整性,而不是提取方法。避免血浆脂质污染后,凝胶过滤血小板的结果与洗涤血小板的结果相关。

相似文献

1
Reconsideration of quantitative phospholipid analysis in human gel-filtered blood platelets.关于人凝胶过滤血小板中磷脂定量分析的重新思考。
Acta Haematol. 1982;67(1):13-9. doi: 10.1159/000207019.
2
Estimation of the phospholipid distribution in the human platelet plasma membrane based on the effect of phospholipase A2 from Naja nigricollis.基于黑颈眼镜蛇磷脂酶A2的作用对人血小板质膜中磷脂分布的估计
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[Effects of 4 weeks' intake of polyunsaturated fatty acid ethylester rich in eicosapentaenoic acid (ethylester) on plasma lipids, plasma and platelet phospholipid fatty acid composition and platelet aggregation; a double blind study].
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Phospholipids released from activated platelets improve platelet aggregation and endothelial cell migration.从活化血小板释放的磷脂可改善血小板聚集和内皮细胞迁移。
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1
The effects of bisantrene on human platelets.比生群对人血小板的作用。
Invest New Drugs. 1986;4(2):119-25. doi: 10.1007/BF00194590.