Harris C, Roth S, Schmid F R, Anderson B
Immunol Commun. 1981;10(7):601-9. doi: 10.3109/08820138109050713.
Fibronectin was shown to bind to C1q using alkaline phosphatase conjugated fibronectin and C1q coated polystyrene tubes. The binding of the alkaline phosphatase conjugated fibronectin to C1q was dose dependent and inhibited by fibronectin and by the sulfated polymers heparin and chondroitin sulfate. The fibronectin interaction was inhibited only slightly by gelatin indicating that the fibronectin-gelatin interaction was different from that with C1q. Heat aggregated IgG blocked the binding of fibronectin to C1q and fibronectin inhibited the binding of aggregated IgG to C1q. These results suggest that fibronectin may be a factor affecting the determination of immune complexes in serum specimens by C1q binding assays.
使用碱性磷酸酶偶联的纤连蛋白和包被有C1q的聚苯乙烯管，结果显示纤连蛋白能与C1q结合。碱性磷酸酶偶联的纤连蛋白与C1q的结合呈剂量依赖性，并受到纤连蛋白以及硫酸化聚合物肝素和硫酸软骨素的抑制。明胶仅轻微抑制纤连蛋白的相互作用，这表明纤连蛋白与明胶的相互作用不同于其与C1q的相互作用。热聚集的IgG可阻断纤连蛋白与C1q的结合，而纤连蛋白可抑制聚集的IgG与C1q的结合。这些结果表明，纤连蛋白可能是影响通过C1q结合试验测定血清标本中免疫复合物的一个因素。
