非免疫性IgG通过二硫键重排与精子表面结合。
Non-immune IgG binding to the surface of spermatozoa by disulphide rearrangement.
作者信息
Richards J M, Witkin S S
出版信息
Clin Exp Immunol. 1984 Nov;58(2):493-501.
Abstract
The mechanism whereby immunoglobulin binds to the surface of spermatozoa was investigated. IgG reacts with spermatozoa non-specifically via the Fc portion of the molecule, while both IgM and IgA are completely unreactive. This selective binding of IgG is non-competitive, resistant to heat, proteases and non-ionic detergents and is not mediated by an Fc receptor on the sperm surface. Instead, evidence is presented that IgG covalently binds to spermatozoa by an exchange reaction between labile disulphides on both the sperm surface and IgG-Fc. It is calculated that there are 3 X 10(11) exchangeable disulphide bonds per bull spermatozoon.
摘要
对免疫球蛋白与精子表面结合的机制进行了研究。IgG通过分子的Fc部分与精子发生非特异性反应，而IgM和IgA则完全无反应。IgG的这种选择性结合是非竞争性的，对热、蛋白酶和非离子去污剂具有抗性，且不是由精子表面的Fc受体介导的。相反，有证据表明IgG通过精子表面和IgG-Fc上不稳定二硫键之间的交换反应与精子共价结合。据计算，每头公牛精子有3×10¹¹个可交换二硫键。
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