Calamai E G, Beller D I, Unanue E R
J Immunol. 1982 Apr;128(4):1692-4.
The expression of I region-associated (Ia) antigens was studied in macrophages derived from bone marrow cell precursors cultured in L cell-conditioned medium (LCM) as a selective growth stimulator. Very few macrophages expressed Ia either while growing actively in LCM or when quiescent, after the removal of LCM from the culture. The addition of T cell lymphokines, however, stimulated the biosynthesis and membrane expression of Ia. Uptake of bacteria by the macrophage during exposure to the lymphokines produced a synergistic increase in Ia expression. The lymphokine-induced stimulation of Ia was reduced by the simultaneous presence of LCM, suggesting that growth and differentiative stimuli have opposing effects on Ia induction. All macrophage colonies derived from single precursors contained cells that could be induced to express Ia.
在以L细胞条件培养基（LCM）作为选择性生长刺激剂培养的骨髓细胞前体来源的巨噬细胞中，研究了I区相关（Ia）抗原的表达。在LCM中活跃生长时或从培养物中去除LCM后静止时，很少有巨噬细胞表达Ia。然而，添加T细胞淋巴因子可刺激Ia的生物合成和膜表达。在暴露于淋巴因子期间巨噬细胞对细菌的摄取导致Ia表达协同增加。LCM同时存在会降低淋巴因子诱导的Ia刺激，这表明生长和分化刺激对Ia诱导具有相反的作用。来自单个前体的所有巨噬细胞集落都含有可被诱导表达Ia的细胞。
