Scher M G, Unanue E R, Beller D I
J Immunol. 1982 Jan;128(1):447-50.
We have evaluated some of the conditions regulating the selective augmentation of the Ia-positive macrophage population within immunologically induced exudates. Antigen-stimulated T cells secrete a protein referred to as macrophage- (Ia-positive) recruiting factor (MIRF), which when injected i.p. stimulates a 10- to 20-fold increase in the number of Ia-positive exudate macrophages. This response is totally abrogated when mice are lethally irradiated before injection of MIRF or immune T cells. Adoptive transfer of bone marrow cells to irradiated mice substantially restores their ability to respond to the immunologic stimuli, even if the transferred bone marrow has itself been depleted of Ia-positive cells. It was also found that the high level of Ia-positive macrophages induced by MIRF requires a renewable stem cell source in order to be maintained. Finally, even when macrophages were elicited by injecting thioglycollate before irradiation, Ia-positive cells were not induced in response to MIRF. These findings suggest that the target of MIRF in vivo may be restricted to a developmentally young cell within or recently derived from a stem cell compartment such as the bone marrow, and that Ia-positive and Ia-negative macrophages ultimately derive from a potentially common Ia-negative stem cell.
我们已经评估了一些调节免疫诱导渗出物中Ia阳性巨噬细胞群体选择性增加的条件。抗原刺激的T细胞分泌一种称为巨噬细胞（Ia阳性）募集因子（MIRF）的蛋白质，腹腔注射该因子可刺激Ia阳性渗出巨噬细胞数量增加10至20倍。当在注射MIRF或免疫T细胞之前对小鼠进行致死性照射时，这种反应完全消除。将骨髓细胞过继转移到受照射的小鼠中，即使转移的骨髓本身已耗尽Ia阳性细胞，也能基本恢复它们对免疫刺激的反应能力。还发现，MIRF诱导的高水平Ia阳性巨噬细胞需要可再生的干细胞来源才能维持。最后，即使在照射前通过注射巯基乙酸盐引发巨噬细胞，也不会因MIRF而诱导出Ia阳性细胞。这些发现表明，MIRF在体内的靶标可能仅限于骨髓等干细胞区室中或最近源自干细胞区室的发育年轻细胞，并且Ia阳性和Ia阴性巨噬细胞最终源自潜在的共同Ia阴性干细胞。
