Neubauer R H, Levy R, Strnad B C, Rabin H
J Immunogenet. 1981 Dec;8(6):433-42. doi: 10.1111/j.1744-313x.1981.tb00950.x.
The phylogenetic distribution of antigens present on human lymphocytes was investigated by incubating human or simian cells with murine anti-human monoclonal antibodies and then determining the level of reactivity with a radiolabelled anti-murine IgG reagent. The monoclonal antibodies used were specific for a T-cell antigen, lymphoid and lymphoid:myeloid antigens, Ia antigens, and beta 2 microglobulin. The cells examined included B- and T-lymphoblastoid cell lines and fresh peripheral blood lymphocytes separated by sheep erythrocyte rosetting into T-cell and non T-cell fractions. Results of these studies showed that the antibodies gave complete cross-reactivity with gorilla and chimpanzee cells while B-cell lines of orangutan origin had lost lymphoid and beta 2 microglobulin markers. Gibbon cells and cells of Old World and New World monkeys reacted strongly only with monoclonal antibodies against Ia antigenic determinants. These Ia antigens were found on the non T-cell fraction of fresh peripheral lymphocytes, on B-cell lines and on some virus induced T-cell tumour lines. Immunoprecipitation analysis using the anti-Ia antibodies showed a degree of molecular diversity on owl monkey and marmoset cells compared to the Ia antigens associated with human cells.
通过将人或猿猴细胞与鼠抗人单克隆抗体孵育，然后用放射性标记的抗鼠IgG试剂测定反应水平，研究了人淋巴细胞上存在的抗原的系统发育分布。所用的单克隆抗体对T细胞抗原、淋巴细胞和淋巴细胞：髓细胞抗原、Ia抗原以及β2微球蛋白具有特异性。所检测的细胞包括B和T淋巴母细胞系以及通过绵羊红细胞花环法分离成T细胞和非T细胞组分的新鲜外周血淋巴细胞。这些研究结果表明，这些抗体与大猩猩和黑猩猩细胞具有完全交叉反应，而猩猩来源的B细胞系已失去淋巴细胞和β2微球蛋白标记。长臂猿细胞以及旧世界和新世界猴的细胞仅与针对Ia抗原决定簇的单克隆抗体发生强烈反应。这些Ia抗原存在于新鲜外周淋巴细胞的非T细胞组分、B细胞系以及一些病毒诱导的T细胞肿瘤系上。与人类细胞相关的Ia抗原相比，使用抗Ia抗体的免疫沉淀分析显示夜猴和狨猴细胞上存在一定程度的分子多样性。
