Koff W C, Caplan F R, Case S, Halstead S B
Clin Exp Immunol. 1983 Aug;53(2):272-80.
The suitability of owl monkeys as experimental models to study the cellular immune response to respiratory syncytial virus (RSV) infection was examined. Seronegative owl monkeys inoculated intranasally with RSV shed large quantities of virus and developed clinically evident upper respiratory disease. RSV infected monkeys had significant lymphoproliferative responses to RSV antigen by 4 weeks post-infection. In contrast, no positive blastogenic responses were elicited during the acute phase of illness. An in vitro 51Cr release assay was developed to study owl monkey antibody-dependent cellular cytoxicity (ADCC) against RSV infected Hep-2 cells. Peripheral blood mononuclear cells from owl monkeys in the presence of RSV specific antibody caused lysis of RSV infected target cells. The effector cell for ADCC was found to be non-adherent to plastic. The owl monkey RSV ADCC system was found to closely parallel RSV specific ADCC observed with human effector cells. In addition, it was found that heterologous matches of human effector cells with monkey sera and monkey effector cells with human sera were equally efficient in mediating RSV specific ADCC. These studies demonstrate the value of the owl monkey as a model to study the pathogenesis or RSV infections.
研究了夜猴作为研究呼吸道合胞病毒（RSV）感染细胞免疫反应实验模型的适用性。血清阴性的夜猴经鼻内接种RSV后，排出大量病毒，并出现临床上明显的上呼吸道疾病。RSV感染的猴子在感染后4周对RSV抗原产生显著的淋巴细胞增殖反应。相比之下，在疾病急性期未引发阳性的增殖反应。开发了一种体外51Cr释放试验，以研究夜猴针对RSV感染的Hep-2细胞的抗体依赖性细胞毒性（ADCC）。在RSV特异性抗体存在的情况下，来自夜猴的外周血单个核细胞导致RSV感染的靶细胞裂解。发现ADCC的效应细胞不粘附于塑料。发现夜猴RSV ADCC系统与用人效应细胞观察到的RSV特异性ADCC密切相似。此外，还发现人效应细胞与猴血清以及猴效应细胞与人类血清的异种匹配在介导RSV特异性ADCC方面同样有效。这些研究证明了夜猴作为研究RSV感染发病机制模型的价值。
