Kanke Y, Doller H J, Segen B J, Gibson K D
Teratology. 1982 Feb;25(1):81-8. doi: 10.1002/tera.1420250111.
4-Methylumbelliferyl beta-D-xyloside was administered to 9-day-old chick embryos, and the morphological and chemical changes in the embryo were followed daily. Increases in wet weight, Na and Cl content, and visible edema were detectable at 10 days and fully apparent at 11 days. Dry weight increased to the same extent in control and treated embryos for four days, but then diverged. The degree of sulfation of chondroitin sulfate was slightly less in treated than control embryos at 10 days, and reached a steady low value at 11 days. Analysis of glycosaminoglycans in skin, muscle, and aorta showed an increase in chondroitin and its sulfates in the two former tissues but not the latter. In muscle and aorta, the degree of sulfation of chondroitin sulfate was markedly reduced; but in skin the results suggested a more complex picture in which the normal metabolism of glycosaminoglycans was altered. A possible physiological role is suggested for chondroitin sulfate in embryonic soft tissues.
将4-甲基伞形基-β-D-木糖苷施用于9日龄鸡胚，并每天跟踪胚胎的形态和化学变化。在第10天可检测到湿重、钠和氯含量增加以及可见水肿，在第11天则完全显现。在四天内，对照胚胎和处理过的胚胎干重增加程度相同，但随后出现差异。在第10天，处理过的胚胎中硫酸软骨素的硫酸化程度略低于对照胚胎，并在第11天达到稳定的低值。对皮肤、肌肉和主动脉中的糖胺聚糖分析表明，前两个组织中软骨素及其硫酸盐增加，而主动脉中未增加。在肌肉和主动脉中，硫酸软骨素的硫酸化程度明显降低；但在皮肤中，结果表明情况更为复杂，其中糖胺聚糖的正常代谢发生了改变。有人提出硫酸软骨素在胚胎软组织中可能具有生理作用。
