Lewin L M, Nevo Z, Gabsu A, Weissenberg R
Int J Androl. 1982 Feb;5(1):37-44. doi: 10.1111/j.1365-2605.1982.tb00231.x.
Only a very small portion (4-7%) of the hyaluronidase of rat sperm obtained from the caput or cauda epididymis was related during incubation in capacitation medium for up to 24 h at 37 degree C. A portion of the cell-associated hyaluronidase was accessible to external substrates, allowing sperm suspensions to lyse glycosaminoglycans of cumulus clots rapidly, even without capacitation. This process appears to represent the employment of an enzyme bound to the sperm cell, analogous to the use of solid-phase enzymes in current enzyme technology.
从附睾头或附睾尾获取的大鼠精子中的透明质酸酶，在37℃的获能培养基中孵育长达24小时期间，只有非常小的一部分（4-7%）会释放出来。一部分与细胞相关的透明质酸酶可接触到外部底物，这使得精子悬液能够迅速溶解卵丘凝块中的糖胺聚糖，即使在没有获能的情况下也是如此。这一过程似乎代表了一种与精子细胞结合的酶的作用，类似于当前酶技术中对固相酶的使用。
