Jakschik B A, Harper T, Murphy R C
J Biol Chem. 1982 May 25;257(10):5346-9.
The homogenate of rat basophilic leukemia cells, when incubated with arachidonic acid, glutathione, and calcium, formed 3 isomers of 5,12-dihydroxyeicosatetraenoic acid and 2 isomers of 5,6-dihydroxyeicosatetraenoic acid, as well as leukotriene (LT) C4 and D4. The products were identified by high pressure liquid chromatography, ultraviolet spectral analysis, co-migration with standards, bioassay, and gas chromatography-mass spectrometry. The enzymes responsible for the formation of LTC4 and LTD4 from LTA4 were found in the 10,000 x g pellet and, therefore, appear to be particulate. The possibility that these enzymes are bound to the cell membrane suggest that the formation of these leukotrienes might be important in the basophil and mast cells release reaction.
大鼠嗜碱性白血病细胞匀浆在与花生四烯酸、谷胱甘肽和钙一起孵育时，形成了5,12-二羟基二十碳四烯酸的3种异构体和5,6-二羟基二十碳四烯酸的2种异构体，以及白三烯（LT）C4和D4。这些产物通过高压液相色谱、紫外光谱分析、与标准品共迁移、生物测定和气相色谱-质谱法进行鉴定。负责从LTA4形成LTC4和LTD4的酶存在于10,000×g沉淀中，因此似乎是颗粒性的。这些酶与细胞膜结合的可能性表明，这些白三烯的形成可能在嗜碱性粒细胞和肥大细胞释放反应中起重要作用。
