Chilton F H, O'Flaherty J T, Walsh C E, Thomas M J, Wykle R L, DeChatelet L R, Waite B M
J Biol Chem. 1982 May 25;257(10):5402-7.
1-O-Alkyl-2-O-acetyl-sn-glycero-3-phosphocholine (AAGPC) triggered the release of [3H]arachidonate but not [14C]stearate from cellular phospholipids in cytochalasin B-treated rabbit polymorphonuclear leukocytes. Concentrations of AAGPC up to 20 nM caused a dose-dependent release and subsequent metabolism of the released [3H]arachidonic acid. Most of the release of the [3H]arachidonate had taken place within the first 2 min of stimulation. Phosphatidylinositol and phosphatidylcholine served as the sources of [3H]arachidonate with about 50% of the label coming from each pool. Challenge of cytochalasin B-treated polymorphonuclear leukocytes with AAPGC led to the production of [3H]hydroxyeicosatetraenoic acids and [3H]dihydroxyeicosatetraenoic acids. No significant production of [3H]prostaglandins or [3H]thromboxanes was detected. AAGPC also caused a dose-dependent degranulation of cytochalasin B-treated rabbit polymorphonuclear leukocytes as shown by the release of beta-glucuronidase and lysozyme. Both the AAGPC-stimulated production of arachidonate metabolites and the degranulation response were blocked by eicosatetraynoic acid and non-dihydroguaiaretic acid at similar inhibitor concentrations. These findings suggest the bioactions of AAGPC on polymorphonuclear leukocytes may be mediated by the release of arachidonic acid and the production of mono- and dihydroxyeicosatetraenoic acids.
1-O-烷基-2-O-乙酰基-sn-甘油-3-磷酸胆碱（AAGPC）可促使细胞松弛素B处理的兔多形核白细胞从细胞磷脂中释放[3H]花生四烯酸，但不能释放[14C]硬脂酸。高达20 nM的AAGPC浓度可引起剂量依赖性释放以及随后释放的[3H]花生四烯酸的代谢。[3H]花生四烯酸的大部分释放在刺激的前2分钟内发生。磷脂酰肌醇和磷脂酰胆碱作为[3H]花生四烯酸的来源，每个池约有50%的标记物来自其中。用AAPGC刺激细胞松弛素B处理的多形核白细胞会导致[3H]羟基二十碳四烯酸和[3H]二羟基二十碳四烯酸的产生。未检测到[3H]前列腺素或[3H]血栓素的显著产生。AAGPC还导致细胞松弛素B处理的兔多形核白细胞发生剂量依赖性脱颗粒，如β-葡萄糖醛酸酶和溶菌酶的释放所示。在相似的抑制剂浓度下，二十碳四炔酸和非二氢愈创木酸可阻断AAGPC刺激的花生四烯酸代谢产物的产生和脱颗粒反应。这些发现表明，AAGPC对多形核白细胞的生物作用可能是由花生四烯酸的释放以及单羟基和二羟基二十碳四烯酸的产生介导的。
