Stöffler G, Tate W P, Caskey C T
J Biol Chem. 1982 Apr 25;257(8):4203-6.
The peptide chain termination factor 2 (RF2) was covalently linked to Escherichia coli ribosomal proteins with the bifunctional reagent, dimethyl suberimidate. Ribosomal RF2 complexes were identified by immunological and radioimmunological techniques which utilized both antibody specific for individual ribosomal proteins and also for RF2. Efficient cross-linking occurred with proteins L2, L7/L12, and L11 of the large ribosome subunit and to a lesser extent with S6, S17, and S18 of the small subunit. On the basis of these and other data the ribosomal binding domain of RF2 appears to be a small region at the interface between the 30 S and 50 S subunits involving parts of both subunits.
肽链终止因子2（RF2）通过双功能试剂辛二亚氨酸二甲酯与大肠杆菌核糖体蛋白共价连接。利用针对单个核糖体蛋白以及RF2的抗体，通过免疫学和放射免疫技术鉴定核糖体RF2复合物。在大核糖体亚基的L2、L7/L12和L11蛋白上发生了高效交联，在小亚基的S6、S17和S18蛋白上的交联程度较低。基于这些及其他数据，RF2的核糖体结合结构域似乎是30S和50S亚基之间界面处的一个小区域，涉及两个亚基的部分区域。
