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A single mutation in 16S rRNA that affects mRNA binding and translation-termination.

作者信息

Prescott C D, Göringer H U

机构信息

Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin, FRG.

出版信息

Nucleic Acids Res. 1990 Sep 25;18(18):5381-6. doi: 10.1093/nar/18.18.5381.

DOI:10.1093/nar/18.18.5381
PMID:2216710
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC332213/
Abstract

A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli (1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitro technique of toeprinting (2) was used to demonstrate that 30S subunits containing the mutation 726G had an altered binding affinity for mRNA by comparison to the wild type. In addition, expression of the mutant ribosomes in vivo resulted in exclusive suppression of the UGA nonsense codon. This effect was supported by in vitro studies that showed the mutant ribosomes to have an altered binding affinity for Release Factor-2.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/1c94e0641b0b/nar00202-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/521872383238/nar00202-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/8ca87fc7b091/nar00202-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/1c94e0641b0b/nar00202-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/521872383238/nar00202-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/8ca87fc7b091/nar00202-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f3a/332213/1c94e0641b0b/nar00202-0048-a.jpg

相似文献

1
A single mutation in 16S rRNA that affects mRNA binding and translation-termination.
Nucleic Acids Res. 1990 Sep 25;18(18):5381-6. doi: 10.1093/nar/18.18.5381.
2
The involvement of base 1054 in 16S rRNA for UGA stop codon dependent translational termination.16S rRNA中第1054位碱基参与UGA终止密码子依赖性的翻译终止过程。
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3
Ribosomes containing the C1054-deletion mutation in E. coli 16S rRNA act as suppressors at all three nonsense codons.在大肠杆菌16S rRNA中含有C1054缺失突变的核糖体在所有三个无义密码子处都起抑制作用。
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4
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Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.影响UGA（终止密码子）导向的翻译终止的16S核糖体RNA突变。
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Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis.大肠杆菌16S核糖体RNA中第791位的突变影响蛋白质合成起始过程。
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引用本文的文献

1
Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.影响UGA（终止密码子）导向的翻译终止的16S核糖体RNA突变。
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6603-7. doi: 10.1073/pnas.88.15.6603.
2
Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture.双芽巴贝斯虫三个小亚基核糖体RNA基因的序列微异质性：在红细胞培养中的表达
Nucleic Acids Res. 1991 Jul 11;19(13):3641-5. doi: 10.1093/nar/19.13.3641.
3
Conformation and structural fluctuations of a 218 nucleotides long rRNA fragment: 4-thiouridine as an intrinsic photolabelling probe.

本文引用的文献

1
Basepairing potential of the 3' terminus of 16S RNA: dependence on the functional state of the 30S subunit and the presence of protein S21.16S RNA 3' 末端的碱基配对潜力：取决于 30S 亚基的功能状态及蛋白质 S21 的存在。
Nucleic Acids Res. 1981 Mar 25;9(6):1425-44. doi: 10.1093/nar/9.6.1425.
2
Positions of proteins S6, S11 and S15 in the 30 S ribosomal subunit of Escherichia coli.蛋白质S6、S11和S15在大肠杆菌30S核糖体亚基中的位置。
J Mol Biol. 1981 Dec 15;153(3):739-60. doi: 10.1016/0022-2836(81)90416-2.
3
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
一个218个核苷酸长的rRNA片段的构象与结构波动：4-硫尿苷作为一种内在光标记探针
Nucleic Acids Res. 1991 Jul 11;19(13):3653-60. doi: 10.1093/nar/19.13.3653.
4
Homologies between the contiguous and fragmented rRNAs of the two Plasmodium falciparum extrachromosomal DNAs are limited to core sequences.两种恶性疟原虫染色体外DNA的连续和片段化rRNA之间的同源性仅限于核心序列。
Nucleic Acids Res. 1992 Feb 25;20(4):879-87. doi: 10.1093/nar/20.4.879.
5
Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.大肠杆菌16S rRNA中增强和降低抑制性tRNA活性的突变。
Nucleic Acids Res. 1992 Apr 11;20(7):1567-71. doi: 10.1093/nar/20.7.1567.
大肠杆菌核糖体RNA操纵子的基因组织与一级结构
J Mol Biol. 1981 May 15;148(2):107-27. doi: 10.1016/0022-2836(81)90508-8.
4
The function of ribosomal protein S21 in protein synthesis.
Eur J Biochem. 1981 Sep 1;118(3):615-9. doi: 10.1111/j.1432-1033.1981.tb05563.x.
5
Ribosomal proteins cross-linked to peptide chain termination release factor 2.与肽链终止释放因子2交联的核糖体蛋白。
J Biol Chem. 1982 Apr 25;257(8):4203-6.
6
The role of 2-methylthio-N6-isopentenyladenosine in readthrough and suppression of nonsense codons in Escherichia coli.2-甲硫基-N6-异戊烯基腺苷在大肠杆菌中对无义密码子的通读和抑制作用。
Mol Gen Genet. 1983;190(2):289-94. doi: 10.1007/BF00330653.
7
Antibiotic resistance mutations in 16S and 23S ribosomal RNA genes of Escherichia coli.大肠杆菌16S和23S核糖体RNA基因中的抗生素抗性突变
Nucleic Acids Res. 1984 Jun 11;12(11):4653-63. doi: 10.1093/nar/12.11.4653.
8
Nucleotide sequences involved in bacteriophage T4 gene 32 translational self-regulation.参与噬菌体T4基因32翻译自我调控的核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4937-41. doi: 10.1073/pnas.79.16.4937.
9
Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivo.体内参与质粒编码rRNA严格调控的DNA区域。
Cell. 1983 Apr;32(4):1347-54. doi: 10.1016/0092-8674(83)90315-x.
10
Site-directed mutagenesis of ribosomal RNA. Analysis of ribosomal RNA deletion mutants using maxicells.核糖体RNA的定点诱变。使用大细胞分析核糖体RNA缺失突变体。
J Mol Biol. 1982 Aug 15;159(3):417-39. doi: 10.1016/0022-2836(82)90292-3.