7S RNA的核苷酸序列。与Alu DNA和La 4.5S RNA的同源性。
Nucleotide sequence of 7 S RNA. Homology to Alu DNA and La 4.5 S RNA.
作者信息
Li W Y, Reddy R, Henning D, Epstein P, Busch H
出版信息
J Biol Chem. 1982 May 10;257(9):5136-42.
7 S RNA, a component of normal higher eukaryotic cells and several oncornaviruses, was shown to be conserved in evolution (Erikson, E., Erikson, R. L., Henry, B., and Pace, N. R. (1973) Virology 53, 40-46). Recently, 7 S RNA was shown to be partially complementary to Alu family DNA sequences (Weiner, A. (1980) Cell 22, 209-218). In the present study the nucleotide sequence of Novikoff hepatoma 7 S RNA was determined to be: (formula, see text) Comparison of 7 S RNA, Alu and B1 family DNA, and La 4.5 S RNA sequences for homologies showed that 1) one-third of 7 S RNA, mainly the 5'-end, was homologous to Alu and B1 family sequences; 2) one 300-nucleotide long Alu family sequence contained two binding sites for 7 S RNA; and 3) the 5'-ends of 7 S RNA and La 4.5 S RNA also had extensive (60%) homologies. A model for the secondary structure of 7 S RNA based on maximal base pairing and preferential nuclease cleavage sites is also presented.
7S RNA是正常高等真核细胞和几种致癌RNA病毒的一个组成部分，已证明它在进化过程中是保守的（埃里克森，E.，埃里克森，R.L.，亨利，B.，和佩斯，N.R.（1973年）《病毒学》53卷，40 - 46页）。最近，已证明7S RNA与Alu家族DNA序列部分互补（韦纳，A.（1980年）《细胞》22卷，209 - 218页）。在本研究中，诺维科夫肝癌7S RNA的核苷酸序列测定如下：（公式，见正文）对7S RNA、Alu和B1家族DNA以及La 4.5S RNA序列进行同源性比较表明：（1）7S RNA的三分之一，主要是5′端，与Alu和B1家族序列同源；（2）一个300个核苷酸长的Alu家族序列含有两个7S RNA结合位点；（3）7S RNA和La 4.5S RNA的5′端也有广泛的（60%）同源性。还提出了一个基于最大碱基配对和优先核酸酶切割位点的7S RNA二级结构模型。
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