与重复DNA同源的诺维科夫肝癌小RNA的特征分析

Characterization of Novikoff hepatoma small RNAs homologous to repetitive DNAs.

作者信息

Reddy R, Henning D, Suh D

机构信息

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.

出版信息

Mol Cell Biochem. 1988 Feb;79(2):125-32. doi: 10.1007/BF02424554.

DOI:10.1007/BF02424554

PMID:3398835

Abstract

Three minor small RNA species from Novikoff hepatoma cells, with homology to repetitive DNA sequences, have been identified and characterized. These small RNAs, designated 5.1S, 6S and T3 RNAs, show homology to Alu 1, Alu 2, and Alu 3 sequences, respectively. 6S and T3 RNAs were found both in the nucleus and cytoplasm, whereas 5.1S RNA was not found in the nucleus. Neural tissues were found to contain a 6S-sized BC1 RNA with homology to I.D. sequences; in contrast, the current study shows that Novikoff hepatoma cells contain a 75-80 nucleotide long (T3) RNA, homologous to I.D. sequences. These data suggest that BC1 and T3 small RNAs, homologous to I.D. sequences, are expressed in a tissue-specific manner. These results also show that in addition to the abundant 7SL, 4.5S and 4.5S1 RNAs having homology to repetitive DNA, Novikoff hepatoma cells also contain several minor small RNAs with homology to repetitive sequences.

摘要

已鉴定并表征了来自诺维科夫肝癌细胞的三种与重复DNA序列具有同源性的微小RNA。这些小RNA分别命名为5.1S、6S和T3 RNA，它们分别与Alu 1、Alu 2和Alu 3序列具有同源性。6S和T3 RNA在细胞核和细胞质中均有发现，而5.1S RNA在细胞核中未被发现。研究发现神经组织含有一种与ID序列具有同源性的6S大小的BC1 RNA；相比之下，当前研究表明诺维科夫肝癌细胞含有一种与ID序列同源的75 - 80个核苷酸长的（T3）RNA。这些数据表明，与ID序列同源的BC1和T3小RNA以组织特异性方式表达。这些结果还表明，除了与重复DNA具有同源性的丰富的7SL、4.5S和4.5S1 RNA外，诺维科夫肝癌细胞还含有几种与重复序列具有同源性的微小RNA。