Whitham M, Nixon J E, Sinnhuber R O
J Natl Cancer Inst. 1982 Apr;68(4):623-8.
The in vivo binding of aflatoxin B1 (AFB1) to liver DNA was measured in rainbow trout subjected to various regimens and in coho salmon to determine if binding would correlate with tumor incidence and initiation of hepatocellular carcinoma. Establishment of time-course binding parameters showed no significant difference between 4, 12, 24, or 48 hours. Doses of 5, 25, 100, and 300 micrograms AFB1/kg body weight produced an almost linear increase in binding with increasing dose. Compared to that of controls, AFB1-DNA binding was twofold lower in beta-naphthoflavone-treated trout and twentyfold lower in coho salmon. These two comparisons reflected a decrease in the effective activation and binding of AFB1, correlated with tumor incidence, and are believed to correlate with a decrease in the initiation of hepatocellular carcinoma. AFB1 binding was not significantly altered by dietary protein or cyclopropenoid fatty acids, which are thought to increase hepatocellular carcinoma incidence through promotional effects. The data were consistent with the view that in vivo binding of AFB1 to liver DNA is a measure of cancer inititation by AFB1 and correlated with the incidence of hepatocellular carcinoma in salmonid fish.
在接受不同处理方案的虹鳟鱼和银大麻哈鱼体内测量黄曲霉毒素B1（AFB1）与肝脏DNA的结合情况，以确定这种结合是否与肿瘤发生率以及肝细胞癌的起始相关。时间进程结合参数的确定显示，4、12、24或48小时之间没有显著差异。5、25、100和300微克AFB1/千克体重的剂量随着剂量增加使结合几乎呈线性增加。与对照组相比，β-萘黄酮处理的鳟鱼中AFB1-DNA结合降低了两倍，银大麻哈鱼中降低了二十倍。这两个比较反映了AFB1有效激活和结合的减少，与肿瘤发生率相关，并且被认为与肝细胞癌起始的减少相关。饮食中的蛋白质或环丙烯脂肪酸对AFB1结合没有显著改变，而这些物质被认为通过促进作用增加肝细胞癌发生率。数据与以下观点一致：AFB1在体内与肝脏DNA的结合是AFB1引发癌症的一种衡量指标，并且与鲑科鱼类肝细胞癌的发生率相关。
