Hendricks J D, Sinnhuber R O, Nixon J E, Wales J H, Masri M S, Hsieh D P
J Natl Cancer Inst. 1980 Mar;64(3):523-8.
Aflatoxin Q1 (AFQ1), the major microsomal biotransformation product of aflatoxin B1 (AFB1) formed in vitro by monkey and human liver preparations, was fed to rainbow trout (Salmo gairdneri) in a semipurified diet at levels of 20 ppb for 1 year and 100 ppb for 10 months. As a test for carcinogenicity in hatched fish, it was also used at 1 ppm in a water solution and exposed for 1 hour to fertile trout eggs. The 20-ppb dietary exposure and 1-ppm egg exposure failed to elicit a carcinogenic response; however, the 100-ppb dietary exposure produced a 10.6% incidence of hepatocellular carcinomas at the end of 1 year. Administration of 50 ppm methyl sterculate, a cyclopropenoid fatty acid, in combination with 100 ppb AFQ1 resulted in a synergistic tumor response similar to that previously noted with administration of AFB1 plus aflatoxin M1. The tumor incidence was 89.1% in the fish on the combined diet. These results indicated that AFQ1 was approximately 100 times less carcinogenic than was AFB1. This comparison of carcinogenic potencies was very similar to the comparison of the relative mutagenicities of the two compounds in the Ames bacterial mutagen assay system.
黄曲霉毒素Q1（AFQ1）是黄曲霉毒素B1（AFB1）在体外经猴和人肝脏制剂微粒体生物转化产生的主要产物，以20 ppb的水平添加到虹鳟鱼（Salmo gairdneri）的半纯化饲料中喂养1年，以及以100 ppb的水平喂养10个月。作为对孵化后鱼类致癌性的测试，它还以1 ppm的浓度用于水溶液中，并让受精的鳟鱼卵暴露1小时。20 ppb的饮食暴露和1 ppm的卵暴露未引发致癌反应；然而，100 ppb的饮食暴露在1年末导致肝细胞癌的发生率为10.6%。给予50 ppm的甲基sterculate（一种环丙烯脂肪酸）与100 ppb的AFQ1联合使用，产生了与先前给予AFB1加黄曲霉毒素M1时相似的协同肿瘤反应。联合饲料喂养的鱼的肿瘤发生率为89.1%。这些结果表明，AFQ1的致癌性约为AFB1的100分之一。这种致癌效力的比较与两种化合物在艾姆斯细菌诱变试验系统中的相对诱变性比较非常相似。
