精氨酸血管加压素与去甲肾上腺素对体外松果体吲哚胺合成的相互作用。

Interaction of arginine vasotocin and norepinephrine upon pineal indoleamine synthesis in vitro.

作者信息

Sartin J L, Bruot B C, Orts R J

出版信息

Mol Cell Endocrinol. 1978 Jun;11(1):7-18. doi: 10.1016/0303-7207(78)90028-x.

Abstract

The interaction of arginine vasotocin (AVT) and norepinephrine (NE) upon pineal gland indoleamine synthesis was investigated. Rat pineal glands were incubated for 10 h in Krebs--Ringer bicarbonate plus 2 mg/ml glucose, 1 mg/ml bovine serum albumin, [14C]tryptophan, NE (10(-6) M), and log doses of AVT ranging from 100 ng to 10 microgram. Incubation media were extracted for [14C]serotonin while the other [14C]indoleamines, melatonin, hydroxyindoleacetic acid (HIAA), methoxyindoleacetic acid (MIAA), N-acetylserotonin (NAS), hydroxytryptophol (HTOL), and methoxytryptophol (MTOH) were separated by thin-layer chromatography. Serotonin metabolism was decreased by 0.1 microgram AVT and NAS decreased by 1.0 microgram AVT. Melatonin synthesis was decreased by both 0.1 and 1.0 microgram AVT. AVT also decreased the conversion of [14C]serotonin to MIAA and to HTOL. The data indicates that AVT decreased NE-stimulated pineal indoleamine synthesis in vitro and further suggests that AVT may participate in the intracellular regulation of melatonin synthesis.

摘要

研究了精氨酸血管加压素（AVT）和去甲肾上腺素（NE）对松果体吲哚胺合成的相互作用。将大鼠松果体在含有2mg/ml葡萄糖、1mg/ml牛血清白蛋白、[14C]色氨酸、NE（10(-6)M）以及100ng至10μg对数剂量AVT的 Krebs-Ringer碳酸氢盐溶液中孵育10小时。提取孵育培养基中的[14C]血清素，而其他[14C]吲哚胺、褪黑素、羟基吲哚乙酸（HIAA）、甲氧基吲哚乙酸（MIAA）、N-乙酰血清素（NAS）、羟基色醇（HTOL）和甲氧基色醇（MTOH）则通过薄层色谱法分离。0.1μg AVT可降低血清素代谢，1.0μg AVT可降低NAS。0.1μg和1.0μg AVT均可降低褪黑素合成。AVT还可降低[14C]血清素向MIAA和HTOL的转化。数据表明，AVT在体外可降低NE刺激的松果体吲哚胺合成，进一步表明AVT可能参与褪黑素合成的细胞内调节。