Kühlbrandt W
Ultramicroscopy. 1982;7(3):221-32. doi: 10.1016/0304-3991(82)90169-3.
A contrast variation method is described by which protein and nucleic acid components of nucleoprotein assemblies can be distinguished in the electron microscope. Using contrasting media of variable electron scattering density, the contribution to image contrast of protein or nucleic acid can be matched out. The protein and DNA components of T4 bacteriophages were visualized separately in this way. The distribution of RNA and protein in ribosomes was studied by processing images of crystalline sheets of ribosomes embedded in a range of contrasting media. Projection maps of crystalline ribosomes suggest that the ribosomal RNA is located predominantly in the central region of the particle.
本文描述了一种对比变化方法，利用该方法可在电子显微镜下区分核蛋白组装体中的蛋白质和核酸成分。通过使用具有可变电子散射密度的对比介质，可以消除蛋白质或核酸对图像对比度的贡献。用这种方法分别观察到了T4噬菌体的蛋白质和DNA成分。通过处理嵌入在一系列对比介质中的核糖体晶体片层的图像，研究了核糖体中RNA和蛋白质的分布。核糖体晶体的投影图表明，核糖体RNA主要位于颗粒的中心区域。
