冰中70S大肠杆菌核糖体的三维重建：核糖体RNA的分布
Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA.
作者信息
Frank J, Penczek P, Grassucci R, Srivastava S
机构信息
Wadsworth Center for Laboratories and Research, Albany, New York 12201-0509.
出版信息
J Cell Biol. 1991 Nov;115(3):597-605. doi: 10.1083/jcb.115.3.597.
Abstract
A reconstruction, at 40 A, of the Escherichia coli ribosome imaged by cryo-electron microscopy, obtained from 303 projections by a single-particle method of reconstruction, shows the two subunits with unprecedented clarity. In the interior of the subunits, a complex distribution of higher mass density is recognized, which is attributed to ribosomal RNA. The masses corresponding to the 16S and 23S components are linked in the region of the platform of the small subunit. Thus the topography of the rRNA regions responsible for protein synthesis can be described.
摘要
通过单颗粒重建方法从303个投影获得的大肠杆菌核糖体的40埃分辨率冷冻电子显微镜图像重建，以前所未有的清晰度显示了两个亚基。在亚基内部，可以识别出较高质量密度的复杂分布，这归因于核糖体RNA。与16S和23S组分相对应的质量在小亚基平台区域相连。因此，可以描述负责蛋白质合成的rRNA区域的拓扑结构。
相似文献
1
Three-dimensional reconstruction of the 70S Escherichia coli ribosome in ice: the distribution of ribosomal RNA.冰中70S大肠杆菌核糖体的三维重建：核糖体RNA的分布
J Cell Biol. 1991 Nov;115(3):597-605. doi: 10.1083/jcb.115.3.597.
2
Correlation of the expansion segments in mammalian rRNA with the fine structure of the 80 S ribosome; a cryoelectron microscopic reconstruction of the rabbit reticulocyte ribosome at 21 A resolution.哺乳动物核糖体RNA中扩展片段与80S核糖体精细结构的相关性；兔网织红细胞核糖体在21埃分辨率下的冷冻电子显微镜重建。
J Mol Biol. 1998 Jun 5;279(2):403-21. doi: 10.1006/jmbi.1998.1804.
3
The 70S Escherichia coli ribosome at 23 A resolution: fitting the ribosomal RNA.分辨率为23埃的70S大肠杆菌核糖体：核糖体RNA的拟合
Structure. 1995 Aug 15;3(8):815-21. doi: 10.1016/s0969-2126(01)00216-7.
4
A method for differentiating proteins from nucleic acids in intermediate-resolution density maps: cryo-electron microscopy defines the quaternary structure of the Escherichia coli 70S ribosome.一种在中等分辨率密度图中区分蛋白质和核酸的方法：冷冻电子显微镜确定了大肠杆菌70S核糖体的四级结构。
Structure. 2000 Sep 15;8(9):937-48. doi: 10.1016/s0969-2126(00)00185-4.
5
DNA-hybridization electron microscopy. Localization of five regions of 16 S rRNA on the surface of 30 S ribosomal subunits.DNA杂交电子显微镜技术。16 S核糖体RNA五个区域在30 S核糖体亚基表面的定位。
J Mol Biol. 1990 Feb 20;211(4):897-906. doi: 10.1016/0022-2836(90)90082-W.
6
Controlling orthogonal ribosome subunit interactions enables evolution of new function.控制正交核糖体亚基相互作用可实现新功能的进化。
Nature. 2018 Dec;564(7736):444-448. doi: 10.1038/s41586-018-0773-z. Epub 2018 Dec 5.
7
The Escherichia coli 30S ribosomal subunit; an optimized three-dimensional fit between the ribosomal proteins and the 16S RNA.大肠杆菌30S核糖体亚基；核糖体蛋白与16S RNA之间优化的三维契合。
EMBO J. 1988 May;7(5):1509-13. doi: 10.1002/j.1460-2075.1988.tb02970.x.
8
Prediction of the three-dimensional structure of Escherichia coli 30S ribosomal subunit: a molecular mechanics approach.大肠杆菌30S核糖体亚基三维结构的预测：一种分子力学方法。
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1950-4. doi: 10.1073/pnas.87.5.1950.
9
The three-dimensional structure and function of Escherichia coli ribosomal RNA, as studied by cross-linking techniques.通过交联技术研究大肠杆菌核糖体RNA的三维结构与功能。
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):8-13. doi: 10.1016/0167-4781(90)90133-m.
10
Direct three-dimensional localization and positive identification of RNA helices within the ribosome by means of genetic tagging and cryo-electron microscopy.通过基因标记和冷冻电子显微镜对核糖体中的RNA螺旋进行直接三维定位和阳性鉴定。
Structure. 1999 Dec 15;7(12):1567-73. doi: 10.1016/s0969-2126(00)88347-1.
引用本文的文献
1
A measure for the identification of preferred particle orientations in cryo-electron microscopy data: A simulation study.一种用于识别冷冻电子显微镜数据中首选粒子取向的方法：模拟研究。
Biophys Physicobiol. 2021 Apr 16;18:96-107. doi: 10.2142/biophysico.bppb-v18.011. eCollection 2021.
2
Two Decades of Evolution of Our Understanding of the Transient Receptor Potential Melastatin 2 (TRPM2) Cation Channel.二十年来我们对瞬时受体电位褪黑素2（TRPM2）阳离子通道认识的演变
Life (Basel). 2021 Apr 27;11(5):397. doi: 10.3390/life11050397.
3
Methods and application of coherent X-ray diffraction imaging of noncrystalline particles.非晶态颗粒的相干X射线衍射成像方法及应用
Biophys Rev. 2020 Apr;12(2):541-567. doi: 10.1007/s12551-020-00690-9. Epub 2020 Mar 16.
4
Joachim Frank's Binding with the Ribosome.乔奇姆·弗兰克与核糖体的结合。
Structure. 2019 Mar 5;27(3):411-419. doi: 10.1016/j.str.2018.11.006. Epub 2018 Dec 27.
5
goCTF: Geometrically optimized CTF determination for single-particle cryo-EM.goCTF：用于单颗粒冷冻电镜的几何优化 CTF 确定。
J Struct Biol. 2019 Jan 1;205(1):22-29. doi: 10.1016/j.jsb.2018.11.012. Epub 2018 Nov 26.
6
Combined H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments.结合 H 检测固态 NMR 光谱学和电子晶体学在天然环境中研究分辨率不同的膜蛋白。
Structure. 2018 Jan 2;26(1):161-170.e3. doi: 10.1016/j.str.2017.11.011. Epub 2017 Dec 14.
7
The ribosome and its role in protein folding: looking through a magnifying glass.核糖体及其在蛋白质折叠中的作用：透过放大镜观察。
Acta Crystallogr D Struct Biol. 2017 Jun 1;73(Pt 6):509-521. doi: 10.1107/S2059798317007446. Epub 2017 May 31.
8
Intersubunit Bridges of the Bacterial Ribosome.细菌核糖体的亚基间桥
J Mol Biol. 2016 May 22;428(10 Pt B):2146-64. doi: 10.1016/j.jmb.2016.02.009. Epub 2016 Feb 13.
9
From high symmetry to high resolution in biological electron microscopy: a commentary on Crowther (1971) 'Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs'.生物电子显微镜中从高对称性到高分辨率：对克劳瑟（1971年）“通过电子显微照片的傅里叶合成对球形病毒进行三维重建的程序”的评论
Philos Trans R Soc Lond B Biol Sci. 2015 Apr 19;370(1666). doi: 10.1098/rstb.2014.0345.
10
Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles.利用三万个冷冻电镜颗粒构建出近原子分辨率的核糖体结构。
Elife. 2013 Feb 19;2:e00461. doi: 10.7554/eLife.00461.
本文引用的文献
1
Computer averaging of electron micrographs of 40S ribosomal subunits.40S核糖体亚基电子显微照片的计算机平均化处理。
Science. 1981 Dec 18;214(4527):1353-5. doi: 10.1126/science.7313694.
2
Use of multivariate statistics in analysing the images of biological macromolecules.多元统计在生物大分子图像分析中的应用。
Ultramicroscopy. 1981;6(2):187-94. doi: 10.1016/0304-3991(81)90059-0.
3
Effect of translocation on topology and conformation of anticodon and D loops of tRNAPhe.易位对苯丙氨酸转运核糖核酸反密码子环和D环的拓扑结构及构象的影响
J Mol Biol. 1981 Sep 5;151(1):57-79. doi: 10.1016/0022-2836(81)90221-7.
4
The subunit interface of the Escherichia coli ribosome. Identification of proteins at the interface between the 30 S and 50 S subunits by crosslinking with 2-iminothiolane.大肠杆菌核糖体的亚基界面。通过与2-亚氨基硫杂环戊烷交联鉴定30 S和50 S亚基之间界面处的蛋白质。
J Mol Biol. 1981 Jul 5;149(3):451-76. doi: 10.1016/0022-2836(81)90481-2.
5
Correct codon--anticodon base pairing at the 5'-anticodon position blocks covalent cross-linking between transfer ribonucleic acid and 16S RNA at the ribosomal P site.5'-反密码子位置上正确的密码子-反密码子碱基配对可阻止核糖体P位点处转移核糖核酸与16S RNA之间的共价交联。
Biochemistry. 1981 Feb 3;20(3):552-9. doi: 10.1021/bi00506a017.
6
Topography of RNA in the ribosome: location of the 3'-end of 5 S RNA on the central protuberance of the 50 S subunit.核糖体中RNA的拓扑结构：5S RNA 3′端在50S亚基中央突起上的位置。
FEBS Lett. 1980 Nov 17;121(1):97-100. doi: 10.1016/0014-5793(80)81274-9.
7
Distribution of RNA and protein in crystalline eukaryotic ribosomes.RNA和蛋白质在真核生物晶体核糖体中的分布。
J Mol Biol. 1982 Apr 15;156(3):431-48. doi: 10.1016/0022-2836(82)90259-5.
8
Discrimination of protein and nucleic acids by electron microscopy using contrast variation.利用对比度变化通过电子显微镜鉴别蛋白质和核酸。
Ultramicroscopy. 1982;7(3):221-32. doi: 10.1016/0304-3991(82)90169-3.
9
Specific visualization of ribosomal RNA in the intact ribosome by electron spectroscopic imaging.通过电子光谱成像对完整核糖体中的核糖体RNA进行特异性可视化。
Eur J Cell Biol. 1983 Sep;31(2):334-40.
10
Representation of three-dimensionally reconstructed objects in electron microscopy by surfaces of equal density.通过等密度表面在电子显微镜中对三维重建物体进行表征。
J Microsc. 1984 Oct;136(Pt 1):77-85. doi: 10.1111/j.1365-2818.1984.tb02547.x.
Zotero 插件