患有凝血障碍的患者在乙型肝炎病毒标志物放射免疫分析中的假反应。

False reactions in radioimmunoassay for viral hepatitis B markers in patients suffering from coagulation disorders.

作者信息

Grangeot-Keros L, Lambert T, Dubreuil P, Briantais M J, Pillot J

出版信息

Vox Sang. 1982 Mar;42(3):160-3. doi: 10.1111/j.1423-0410.1982.tb01088.x.

DOI:10.1111/j.1423-0410.1982.tb01088.x

Abstract

Simultaneous occurrence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) has been frequently described in hemophiliac patients. During our working procedures, this phenomenon was also observed. Interference of coagulation disorders with radioimmunoassay (RIA) used in the detection of viral hepatitis B markers was studied. It was shown that, in vivo or in vitro, correction of coagulation disorders led to the disappearance of false positive reactions and, thus, simultaneous presence of HBsAg and anti-HBs never occurred. Confirmatory tests which are usually recommended to control RIA were reviewed and it found that only a completed coagulation of serum by thrombin addition, before performing the test, gave specific results.

摘要

乙肝表面抗原（HBsAg）和乙肝表面抗原抗体（抗-HBs）同时出现的情况在血友病患者中已有频繁报道。在我们的工作过程中，也观察到了这种现象。我们研究了凝血障碍对用于检测乙肝病毒标志物的放射免疫分析（RIA）的干扰。结果表明，无论在体内还是体外，纠正凝血障碍都会导致假阳性反应消失，因此，HBsAg和抗-HBs不会同时出现。我们回顾了通常推荐用于控制RIA的验证试验，发现只有在进行试验前通过添加凝血酶使血清完全凝固，才能得到特异性结果。