Reutgen H
Z Erkr Atmungsorgane. 1982;158(1-2):130-41.
A method is described which allows a rapid primary culture of mycobacteria. The basic principle is a rotating incubator fitted with special cultures tubes and a synthetic liquid culture medium. So it is possible to accelerate the growth and multiplication and to get instance an average generation time of 10 hours in M. tuberculosis. After conventional decontamination of the sputa with Nekal-BX and an incubation time of 1, 2 and 4 weeks the grown mycobacteria are proved by microscopy with a special fixation technique. Acridin-orange is used for staining. The conditions for application of the rapid culture method are discussed.
本文描述了一种可实现分枝杆菌快速原代培养的方法。其基本原理是使用配备特殊培养管和合成液体培养基的旋转培养箱。这样就能够加速生长和繁殖，例如在结核分枝杆菌中可实现平均10小时的代时。在用Nekal-BX对痰液进行常规净化处理并经过1、2和4周的培养时间后，通过一种特殊的固定技术进行显微镜检查来鉴定生长出的分枝杆菌。使用吖啶橙进行染色。文中还讨论了快速培养方法的应用条件。
