Reutgen H, Sesser I, Schubert K, Bennert G, Makarewitsch N
Z Erkr Atmungsorgane. 1980;155(1):59-65.
The optimal qualitative and quantitative identification by microscopy of mycobacteria cultivated in liquid medium in including several problems. Acridine-orange was used for staining in all investigations. Polyvinyl-alcohol (1--2.5%) proved to be the best means for fixing mycobacteria culture suspensions on slides. A reliable sterilization of the sides was possible with peracetic acid (3%). Tween 80 in the culture medium did not impair the adherence of the fixed suspension on the slide under these conditions. Mycobacteria in a suspension of 10(-4) mg (wet weight) per ml could be still found by microscopy with this procedure.
通过显微镜对液体培养基中培养的分枝杆菌进行最佳定性和定量鉴定存在若干问题。在所有研究中均使用吖啶橙进行染色。聚乙烯醇（1%-2.5%）被证明是将分枝杆菌培养悬液固定在载玻片上的最佳方法。用过氧乙酸（3%）可对载玻片进行可靠的消毒。在这些条件下，培养基中的吐温80不会损害固定悬液在载玻片上的附着。采用此方法，每毫升含10⁻⁴毫克（湿重）的悬液中的分枝杆菌仍可通过显微镜检测到。
