Klesov A A, Grigorash S Iu
Biokhimiia. 1982 Mar;47(3):409-18.
It was shown that one of the cellulase components, i.e. cellobiase, can be adsorbed on cellulose surface with the concomitant decrease of activity (by 10 times and more). The specific activity of the adsorbed cellobiase depends on the enzyme concentration in the adsorption layer and is increased with the increase in the surface concentration of cellobiase. It was found that variations in the amount of non-soluble cellulose and the corresponding changes in cellobiase activity in the system (as a result of the adsorption) can lead to a certain alteration in the shape of the kinetic curves for formation of intermediate cellobiose, which in its turn controls the rate of formation of the end product, i.e. glucose. Thus, the substrate surface causes a regulatory effect on the rate and kinetic mechanism of the enzymatic conversion of cellulose to glucose due to the adsorption effects.
结果表明，纤维素酶的一种组分，即纤维二糖酶，可吸附在纤维素表面，同时活性降低（降低10倍及更多）。吸附的纤维二糖酶的比活性取决于吸附层中的酶浓度，并随纤维二糖酶表面浓度的增加而增加。研究发现，体系中不溶性纤维素量的变化以及纤维二糖酶活性的相应变化（由于吸附作用）可导致中间产物纤维二糖形成动力学曲线形状的一定改变，这进而控制了终产物即葡萄糖的形成速率。因此，由于吸附作用，底物表面对纤维素酶促转化为葡萄糖的速率和动力学机制产生调节作用。
