Wynne E, Slocombe J O, Wilkie B N
Can J Comp Med. 1981 Jul;45(3):259-65.
Rabbit antisera were prepared against veronal buffered saline extracts of L4 and L5 Strongylus vulgaris, adult S. vulgaris and adult Strongylus equinus retrieved from naturally infected horses. In agar gel diffusion with these antisera, adult S vulgaris and S. equinus each appeared to have at least one unique antigen; larval S. vulgaris appeared to have two species-specific and two stage-specific antigens. There were several common antigens. Excretory and secretory products were collected also from L4 and L5 an maintained over several days in tissue culture fluid. In agar gel diffusion against the above rabbit antisera, a stage-specific antigen was found also in excretory and secretory products. In addition, excretory and secretory products had three antigens in common with adult and larval S. vulgaris, but only one of these was common to adult S. equinus. The excretory and secretory products appear, therefore, to have two species-specific and one stage-specific antigens.
用从自然感染马体内获取的普通圆线虫L4和L5、普通圆线虫成虫以及马圆线虫成虫的巴比妥缓冲盐水提取物制备兔抗血清。在与这些抗血清的琼脂凝胶扩散试验中，普通圆线虫成虫和马圆线虫成虫似乎各有至少一种独特抗原；普通圆线虫幼虫似乎有两种种特异性抗原和两种阶段特异性抗原。存在几种共同抗原。还从普通圆线虫L4和L5收集了排泄和分泌产物，并在组织培养液中保存了数天。在针对上述兔抗血清的琼脂凝胶扩散试验中，在排泄和分泌产物中也发现了一种阶段特异性抗原。此外，排泄和分泌产物与普通圆线虫成虫和幼虫有三种共同抗原，但其中只有一种是马圆线虫成虫所共有的。因此，排泄和分泌产物似乎有两种种特异性抗原和一种阶段特异性抗原。
