Corbett J, Albro P W, Chae K, Jordan S
Chem Biol Interact. 1982 Apr;39(3):331-8. doi: 10.1016/0009-2797(82)90049-7.
Male CD rats received i.p. injections of 2,3,4,5,3',4',5'-heptachlorobiphenyl (7CB) under conditions resulting in induction of both benzphetamine demethylase (cytochrome P-450-dependent) and ethoxyresorufin deethylase (cytochrome P-448-dependent) activities. To test the possibility that one class of induction (P-450) was due to the parent compound and the other (P-448) to a metabolite, liver, adipose, bile, urine and feces were analyzed for the presence of 3,4,5,3',4',5'-hexachlorobiphenyl (6CB) (the major photolysis product of 7CB) and/or 3-hydroxy-3,4,5,3',4',5'-hexachlorobiphenyl (OH-6CB), another known P-448 inducer. To allow for the possibility that 6CB might be a transient intermediate. The results supported the hypothesis that 7CB is a true 'mixed inducer', nor requiring metabolic alteration for its dual activity.
雄性CD大鼠腹腔注射2,3,4,5,3',4',5'-七氯联苯（7CB），注射条件能诱导苯丙胺脱甲基酶（细胞色素P-450依赖型）和乙氧基试卤灵脱乙基酶（细胞色素P-448依赖型）的活性。为了检验一类诱导作用（P-450）是由母体化合物引起而另一类（P-448）是由代谢物引起的可能性，对肝脏、脂肪、胆汁、尿液和粪便进行分析，检测其中是否存在3,4,5,3',4',5'-六氯联苯（6CB）（7CB的主要光解产物）和/或3-羟基-3,4,5,3',4',5'-六氯联苯（OH-6CB）（另一种已知的P-448诱导剂）。考虑到6CB可能是一种短暂的中间体。结果支持了7CB是一种真正的“混合诱导剂”这一假说，其双重活性不需要代谢改变。
