Kim Y D, Weber G F, Tomita J T, Hirata A A
Clin Chem. 1982 May;28(5):1133-6.
In measuring total galactosyltransferase activity in the pleural effusions from patients with benign or malignant diseases, we found no significant difference between the two groups (p greater than 0.05). However, a small amount of a galactosyltransferase variant, GT(l), could be separated from other galactosyltransferase enzymes in malignant pleural effusions by anion-exchange chromatography (DEAE-cellulose) with a buffer of low ionic strength. Other galactosyltransferases were eluted from the column with buffer of higher ionic strength. Using a mini-column method, we detected GT(l) enzyme in 19 of 26 specimens fro cancer patients, as compared with eight of 25 specimens from patients with benign disorders. The appearance of GT(l) enzyme in pleural effusion may be a tumor-associated phenomenon.
在测量良性或恶性疾病患者胸腔积液中的总半乳糖基转移酶活性时，我们发现两组之间无显著差异（p大于0.05）。然而，通过使用低离子强度缓冲液的阴离子交换色谱法（DEAE-纤维素），可以从恶性胸腔积液中的其他半乳糖基转移酶中分离出少量的半乳糖基转移酶变体GT(l)。其他半乳糖基转移酶用更高离子强度的缓冲液从柱上洗脱。使用微型柱法，我们在26例癌症患者的标本中检测到19例含有GT(l)酶，而25例良性疾病患者的标本中只有8例检测到。胸腔积液中GT(l)酶的出现可能是一种肿瘤相关现象。
