Boyle F A, Cook N D, Peters T J
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Clin Chim Acta. 1988 Mar 15;172(2-3):291-6. doi: 10.1016/0009-8981(88)90335-x.
Cacodylate buffer, frequently used in the assay of galactosyltransferase, causes inhibition of enzyme purified from human pleural effusion. The inhibition is reflected by an increase in the Km (UDP-galactose) and a decrease in Vmax and cannot be reversed by the addition of 2-mercaptoethanol. It is suggested that alternative buffers be used for the study of the enzyme.
常用于半乳糖基转移酶测定的二甲胂酸盐缓冲液会抑制从人胸腔积液中纯化的酶。这种抑制表现为米氏常数（UDP-半乳糖）增加，最大反应速度降低，并且添加2-巯基乙醇不能使其逆转。建议在该酶的研究中使用其他缓冲液。
