克隆于大肠杆菌中的小牛前凝乳酶原cDNA的核苷酸序列。

Nucleotide sequence of calf prorennin cDNA cloned in Escherichia coli.

作者信息

Nishimori K, Kawaguchi Y, Hidaka M, Uozumi T, Beppu T

出版信息

J Biochem. 1982 Mar;91(3):1085-8. doi: 10.1093/oxfordjournals.jbchem.a133758.

DOI:10.1093/oxfordjournals.jbchem.a133758

Abstract

The nucleotide sequence of prorennin (prochymosin) cDNA cloned in E. coli was determined by the technique of Maxam and Gilbert. The longest prorennin cDNA insert in pTACR1 contained the putative signal sequence and the coding sequence for the peptide from the 1st amino acid, Ala (NH2 terminal), to the 296th, Ser, and the other clone pTACR9 contained the coding sequence from the 258th, Asp, to the 365th, Ile (COOH terminal), and the TGA termination codon followed by the 3'-untranslated region. Thus, the whole coding sequence for prorennin was obtained in the pair of pTACR1 and pTACR9.

摘要

采用马克萨姆-吉尔伯特技术测定了克隆于大肠杆菌中的前凝乳酶（前糜蛋白酶）cDNA的核苷酸序列。pTACR1中最长的前凝乳酶cDNA插入片段包含假定的信号序列以及从第1个氨基酸丙氨酸（氨基端）到第296个氨基酸丝氨酸的肽段编码序列，另一个克隆pTACR9包含从第258个氨基酸天冬氨酸到第365个氨基酸异亮氨酸（羧基端）的编码序列，以及TGA终止密码子和其后的3'非翻译区。因此，通过pTACR1和pTACR9这一对克隆获得了前凝乳酶的完整编码序列。