Nishimori K, Kawaguchi Y, Hidaka M, Uozumi T, Beppu T
Gene. 1982 Oct;19(3):337-44. doi: 10.1016/0378-1119(82)90024-5.
An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lacUV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of beta-galactosidase. Synthesis of the fused protein with the expected Mr was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.
构建了一个用于小牛凝乳酶原（前胃蛋白酶）cDNA的表达质粒。该质粒（pCR301）在融合基因前含有lacUV5启动子，其中凝乳酶原cDNA的N端四个氨基酸的密码子被β-半乳糖苷酶的N端十个氨基酸的密码子所取代。在携带pCR301的大肠杆菌中通过免疫学方法检测到了具有预期分子量的融合蛋白的合成。该产物似乎定位于细菌宿主的细胞膜中。
