Vierling J M, Steer C J, Hickman J W, James S P, Jones E A
Gastroenterology. 1978 Sep;75(3):456-61.
The ability of a purified rabbit liver membrane protein that selectively binds desialylated glycoproteins to induce desialylated human peripheral blood lymphocytes to mediate mitogen-induced cellular cytotoxicity has been determined. After short term exposure to purified hepatic binding protein, desialylated, but not intact, lymphocytes exhibited mitogen-induced cellular cytotoxicity against Chang target cells, which do not have exposed hepatic binding protein on their surface membrane. Furthermore, desialylated lymphocytes, in the absence of purified hepatic binding protein, reduced the proportion of isolated rabbit hepatocytes that adhered to plastic to a significantly greater extent than did intact lymphocytes, suggesting that the exposed hepatic binding protein demonstrated on the surface membrane of these target cells is capable of inducing mitogen-induced cellular cytotoxicity. The specific inhibition of this effect by asialo-orosomucoid, but not by intact orosomucoid, indicates that the site involved in the binding of asialo-glycoproteins to hepatic binding protein is probably also responsible for the induction of mitogen-induced cellular cytoxocity. The demonstration that hepatic binding protein, a normal constituent of the surface, membrane of mammalian hepatocytes, can induce mitogen-induced cellular cytotoxicity, suggests that mitogen-induced cellular cytotoxicity may be a mechanism of cellular injury in vivo.
已确定一种能选择性结合去唾液酸化糖蛋白的纯化兔肝膜蛋白，诱导去唾液酸化人外周血淋巴细胞介导有丝分裂原诱导的细胞毒性的能力。短期暴露于纯化的肝结合蛋白后，去唾液酸化而非完整的淋巴细胞表现出对Chang靶细胞的有丝分裂原诱导的细胞毒性，这些靶细胞表面膜上没有暴露的肝结合蛋白。此外，在没有纯化肝结合蛋白的情况下，去唾液酸化淋巴细胞比完整淋巴细胞更显著地降低了附着于塑料的分离兔肝细胞的比例，这表明这些靶细胞表面膜上展示的暴露肝结合蛋白能够诱导有丝分裂原诱导的细胞毒性。去唾液酸血清类黏蛋白而非完整血清类黏蛋白对这种效应的特异性抑制表明，参与去唾液酸化糖蛋白与肝结合蛋白结合的位点可能也负责有丝分裂原诱导的细胞毒性的诱导。哺乳动物肝细胞表面膜的正常成分肝结合蛋白能够诱导有丝分裂原诱导的细胞毒性，这表明有丝分裂原诱导的细胞毒性可能是体内细胞损伤的一种机制。
