连续流血液分离白细胞去除术对人淋巴细胞体外功能的影响。
Influence of continuous-flow blood separator leucophoresis on in vitro functions of human lymphocytes.
作者信息
Siewertsen H C, Peter H H, Kalden J R, Wysk J, Eckert G, Oehl S, Schäfer U W, Deicher H
出版信息
Clin Exp Immunol. 1978 Jun;32(3):381-6.
The influence of continuous-flow blood separator leucophoresis on these in vitro functions of human lymphocytes was studied. In ten separation experiments, lymphocyte functions were examined in the presence and absence of mononuclear phagocytes before, during and at the end of continuous flow leucophoresis. Cell surface markers and effector functions in spontaneous cell-mediated cytotoxicity (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) assays did not change significantly under the conditions of leucophoresis. By contrast, separator lymphocytes exhibited a significantly increased mitogenic response to pokeweed mitogen and PPD. Removal of mononuclear phagocytes corrected for this phenomenon. PHA and Con A responses were not affected by leucophoresis, nor was the number of circulating haemopoietic stem cells (CFU-C) significantly altered during a 4 hr continuous flow blood separator run.
研究了连续流动血液分离器白细胞去除术对人淋巴细胞这些体外功能的影响。在十次分离实验中，在连续流动白细胞去除术之前、期间和结束时，在有和没有单核吞噬细胞的情况下检测淋巴细胞功能。在白细胞去除术条件下，自发细胞介导的细胞毒性（SCMC）和抗体依赖性细胞毒性（ADCC）试验中的细胞表面标志物和效应器功能没有显著变化。相比之下，分离器淋巴细胞对商陆有丝分裂原和结核菌素纯蛋白衍生物表现出明显增强的促有丝分裂反应。去除单核吞噬细胞可纠正此现象。白细胞去除术不影响PHA和Con A反应，在连续流动血液分离器运行4小时期间，循环造血干细胞（CFU-C）数量也未显著改变。
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