Foulkes A, Bradshaw J M, Porter D G
Q J Exp Physiol. 1982 Jan;67(1):81-6. doi: 10.1113/expphysiol.1982.sp002627.
The time that the retention of luminal fluid begins and its release from the uterus in pro-oestrous rats was determined precisely using a dye injection technique. Oestradiol was found to cause dye retention in ovariectomized rats when provided continuously from an implant of 5 mg. Injections of 0.5 microgram/d X 4 were ineffective. Administration or progesterone to rats bearing implants of oestrogen interfered with the ability of the uterus to retain dye. Treatment with relaxin for 28 h prevented retention of eye in pro-oestrous rats whereas treatment at the time of dye injection was without effect. Mechanical stimulation of the cervix did not cause loss of injected dye. It is suggested that the mechanisms of opening of the cervix at coitus and at the end of oestrous may differ.
采用染料注射技术精确测定了动情前期大鼠管腔液潴留开始的时间及其从子宫释放的时间。当从5毫克的植入物中持续提供雌二醇时，发现其会导致去卵巢大鼠出现染料潴留。每天注射0.5微克×4次无效。对植入雌激素的大鼠给予孕酮会干扰子宫保留染料的能力。用松弛素处理28小时可防止动情前期大鼠出现染料潴留，而在注射染料时进行处理则无效。对子宫颈的机械刺激不会导致注入的染料流失。提示性交时和动情期末子宫颈开放的机制可能不同。
