Frohlich J, Hon K, McLeod R
Am J Hum Genet. 1982 Jan;34(1):65-72.
"Rocket" immunoelectrophoresis using specific anti-lecithin: cholesterol acyltransferase (LCAT) antiserum showed no immunoreactive protein in two patients with familial LCAT deficiency. Subnormal quantity of plasma LCAT was found in the maternal grandmother, the parents, and in two of four siblings of the patients (3.3-3.4 mg/l vs. 5.4 +/- 0.5 mg/l in 12 controls). The immunochemical quantitation of the enzyme correlated well (r = .93) with LCAT activity in an artificial substrate assay. These two methods allow detection of heterozygotes for LCAT deficiency.
胆固醇酰基转移酶（LCAT）抗血清进行的“火箭”免疫电泳显示，两名家族性LCAT缺乏症患者中未检测到免疫反应性蛋白。在患者的外祖母、父母以及四名兄弟姐妹中的两名中发现血浆LCAT含量低于正常水平（3.3 - 3.4毫克/升，而12名对照者为5.4±0.5毫克/升）。在人工底物测定中，该酶的免疫化学定量与LCAT活性具有良好的相关性（r = 0.93）。这两种方法能够检测出LCAT缺乏症的杂合子。
