合成基因不同类型胶原蛋白的细胞中赖氨酰羟化酶的催化特性。
Catalytic properties of lysyl hydroxylase from cells synthesizing genetically different collagen types.
作者信息
Puistola U
出版信息
Biochem J. 1982 Jan 1;201(1):215-9. doi: 10.1042/bj2010215.
Abstract
Crude preparations of lysyl hydroxylase were extracted from chick-embryo tendons synthesizing exclusively type I collagen, chick-embryo sterna synthesizing exclusively type II collagen and HT-1080 sarcoma cells synthesizing exclusively type IV collagen. No differences were found in the Km values for Fe2+, 2-oxoglutarate and ascorbate between these three enzymes preparations. Similarly no differences were found in the Km values for type I and type II protocollagens and the rate at which type IV protocollagen is hydroxylated between these enzyme preparations. The extent to which type I protocollagen could be hydroxylated by the three enzymes was likewise identical. These data strongly argue against the existence of collagen-type-specific lysyl hydroxylase isoenzymes.
摘要
从仅合成I型胶原蛋白的鸡胚肌腱、仅合成II型胶原蛋白的鸡胚胸骨以及仅合成IV型胶原蛋白的HT - 1080肉瘤细胞中提取赖氨酰羟化酶的粗制品。在这三种酶制品中，Fe2 +、2 - 氧代戊二酸和抗坏血酸的Km值没有差异。同样，在这些酶制品中，I型和II型原胶原蛋白的Km值以及IV型原胶原蛋白的羟化速率也没有差异。这三种酶对I型原胶原蛋白的羟化程度同样相同。这些数据有力地反驳了存在胶原蛋白类型特异性赖氨酰羟化酶同工酶的观点。
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2
Human lysyl hydroxylase: purification to homogeneity, partial characterization and comparison of catalytic properties with those of a mutant enzyme from Ehlers-Danlos syndrome type VI fibroblasts.
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3
Isolation of lysyl hydroxylase, an enzyme of collagen synthesis, from chick embryos as a homogeneous protein.从鸡胚中分离出赖氨酰羟化酶，一种胶原蛋白合成酶，使其成为一种纯一蛋白质。
Biochem J. 1980 Aug 1;189(2):247-53. doi: 10.1042/bj1890247.
4
Studies on the lysyl hydroxylase reaction. I. Initial velocity kinetics and related aspects.赖氨酰羟化酶反应的研究。I. 初速度动力学及相关方面。
Biochim Biophys Acta. 1980 Jan 11;611(1):40-50. doi: 10.1016/0005-2744(80)90040-6.
5
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