Grossi C E, Zicca A, Leprini A, Cadoni A, Pistoia V, Ferrarini M
Blood. 1982 Jul;60(1):220-7.
Malignant lymphocytes from 30 B-cell chronic lymphocytic leukemia (B-CLL) patients were studied for the cytochemical localization of two acid hydrolases, alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (AT). The large majority of the cells stained for both ANAE and AP in 7 cases, for AP only in 18 cases, and were negative for both the enzymes in 5 cases. Ultrastructural analysis revealed that the cells that displayed more mature morphological features, such as well developed smooth and rough membrane compartments, were those positive for acid hydrolases. That ANAE and AP are expressed by B cells at late stage of maturation was confirmed by the finding that some lymphocytes and all of the plasmacytoid lymphocytes and plasma cells from Walderström's macroglobulinemia, from mixed cryoglobulinemia, and from multiple myeloma patients stained strongly for both ANAE and AP. Using the expression of acid hydrolases and certain ultrastructural features as markers of cell differentiation, it was possible to demonstrate a process of maturation within the single B-CLL clones with accumulation of the cells at stages that differed in the various cases.
对30例B细胞慢性淋巴细胞白血病（B-CLL）患者的恶性淋巴细胞进行研究，以确定两种酸性水解酶——α-萘乙酸酯酶（ANAE）和酸性磷酸酶（AT）的细胞化学定位。在7例患者中，绝大多数细胞同时对ANAE和AP呈阳性染色；18例患者中，细胞仅对AP呈阳性染色；5例患者中，细胞对这两种酶均呈阴性染色。超微结构分析显示，呈现出更成熟形态特征（如发育良好的光滑和粗糙膜性区室）的细胞是酸性水解酶阳性的细胞。来自华氏巨球蛋白血症、混合性冷球蛋白血症和多发性骨髓瘤患者的一些淋巴细胞以及所有浆细胞样淋巴细胞和浆细胞对ANAE和AP均呈强阳性染色，这一发现证实了ANAE和AP在B细胞成熟后期表达。利用酸性水解酶的表达和某些超微结构特征作为细胞分化的标志物，有可能在单个B-CLL克隆中证明一个成熟过程，不同病例中细胞在不同阶段积累。
