胃肠道生长抑素：不同物种中的提取与放射免疫测定
Gastrointestinal somatostatin: extraction and radioimmunoassay in different species.
作者信息
McIntosh C, Arnold R, Bothe E, Becker H, Köbberling J, Creutzfeldt W
出版信息
Gut. 1978 Jul;19(7):655-63. doi: 10.1136/gut.19.7.655.
Abstract
A radioimmunoassay capable of detecting 300 fg somatostatin has been developed and levels of the polypeptide in gastrointestinal tissues from man, dog, and rat have been measured. Rapid freezing of collected samples and careful control of extraction is necessary. Concentrations in different regions of dog antrum (425 +/- 50 to 773 +/- 254 ng/g tissue) are similar to those in antrum from duodenal ulcer patients and control subjects: 614 +/- 125 and 465 +/- 104 ng/g tissue respectively. Levels in histologically normal human pancreas (253 +/- 43 ng/g tissue) are comparable with those in dog pancreas (333 +/- 66 ng/g tissue), whereas in two cases of neonatal hypoglycaemia the concentration exceeded 3000 ng/g tissue. On gel chromatography the majority of immunoreactive somatostatin elutes as the synthetic tetradecapeptide and a small fraction as a larger species.
摘要
已开发出一种能够检测300飞克生长抑素的放射免疫测定法，并已测量了人、狗和大鼠胃肠道组织中该多肽的水平。收集的样品必须快速冷冻并严格控制提取过程。狗胃窦不同区域的浓度（425±50至773±254纳克/克组织）与十二指肠溃疡患者和对照受试者胃窦中的浓度相似，分别为614±125和465±104纳克/克组织。组织学正常的人胰腺中的水平（253±43纳克/克组织）与狗胰腺中的水平（333±66纳克/克组织）相当，而在两例新生儿低血糖症中，浓度超过3000纳克/克组织。在凝胶色谱上，大多数免疫反应性生长抑素以合成十四肽的形式洗脱，一小部分以较大的形式洗脱。
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