Pamperl H, Kleinberger G
Infusionsther Klin Ernahr. 1982 Apr;9(2):86-91. doi: 10.1159/000221324.
Liposomes of Intralipid 20% in four solutions already mixed for total parenteral nutrition (TPN) were studied by means of a transmission electron microscope. Samples of those so-called all-in-one solutions containing all components for TPN in one bottle were taken immediately after the mixing procedure was finished, then after 24 h, 48 h, 1 week, 2 weeks, 1 month and then monthly up to 6 months afterwards. Storage was carried out for one half at 4 degrees C, for the other at 22 degrees C in darkness. The study showed that liposomes of Intralipid 20% in fresh TPN solutions decreased in their medium diameter from 0.8 to 0.6 micrometers. They carried highly osmiophilic inclusion bodies. Prolonged storage led to further alterations, as change of shape, loss of osmiophility, and lastly formation of liposome ghosts. In solutions kept at 4 degrees C those changes developed between the 1st and 2nd month of storage, whereas storage at 22 degrees C was harmful within 48 h. It is recommended, that already prepared TPN solutions should be kept at 3 degrees C and used as soon as possible, until further studies provide exact guidelines for storage time.
采用透射电子显微镜对已混合用于全胃肠外营养（TPN）的四种溶液中20%英脱利匹特脂质体进行了研究。在混合程序完成后，立即从那些在一个瓶子中包含TPN所有成分的所谓一体化溶液中取样，然后在24小时、48小时、1周、2周、1个月后，之后每月取样直至6个月后。一半样品在4℃保存，另一半在22℃黑暗环境中保存。研究表明，新鲜TPN溶液中20%英脱利匹特脂质体的平均直径从0.8微米降至0.6微米。它们带有高度嗜锇性包涵体。长时间储存会导致进一步变化，如形状改变、嗜锇性丧失，最终形成脂质体空壳。在4℃保存的溶液中，这些变化在储存第1个月至第2个月之间出现，而在22℃保存48小时内就会产生有害影响。建议已配制好的TPN溶液应保存在3℃并尽快使用，直到进一步研究提供确切的储存时间指南。
