Hayes R L, Claman H N
J Immunol. 1982 Jul;129(1):232-5.
Alloreactive cytotoxic cells (CTL) are subject to regulation. These experiments were designed to study the in vivo down-regulation of CTL. Mice injected with allogeneic lymphoid cells (responders) develop CTL that are assayed in vitro by their ability to lyse 51Cr-labeled target cells of an appropriate H-2 haplotype. However, if CBA/J responder mice (H-2k, mls d) are pretreated with spleen cells from C3H/HeN mice, which are H-2 compatible (H-2k) but Mis- and minor antigen-disparate (mis c), there is virtually complete inhibition of the ability to develop CTL against A/J cells (H-2a, mls c) in vivo. In a similar manner, pretreatment of CBA/J responders with B10.BR (H-2k, mls b) cells resulted in the inhibition of the CTL response against B10.A(4R) (H-2h4, mls b). The state of CTL responsiveness appears to be H-2 restricted and requires that the allogeneic cells used for immunization share minor histocompatibility antigens with the strain of mouse selected for pretreatment.
同种异体反应性细胞毒性细胞（CTL）受到调控。这些实验旨在研究CTL在体内的下调情况。注射同种异体淋巴细胞（反应细胞）的小鼠会产生CTL，通过其裂解适当H-2单倍型的51Cr标记靶细胞的能力在体外进行检测。然而，如果用C3H/HeN小鼠的脾细胞对CBA/J反应小鼠（H-2k，mls d）进行预处理，C3H/HeN小鼠与CBA/J小鼠H-2相容（H-2k）但次要组织相容性抗原和微小抗原不同（mis c），那么在体内针对A/J细胞（H-2a，mls c）产生CTL的能力几乎会完全受到抑制。以类似的方式，用B10.BR（H-2k，mls b）细胞对CBA/J反应小鼠进行预处理，会导致针对B10.A(4R)（H-2h4，mls b）的CTL反应受到抑制。CTL反应性状态似乎受到H-2限制，并且要求用于免疫的同种异体细胞与选择用于预处理的小鼠品系共享次要组织相容性抗原。
