Whisler R L, Newhouse Y G, Lachman L B
J Immunol. 1982 Aug;129(2):455-60.
The abilities of human peripheral blood mononuclear-phagocyte (M phi) subpopulations and of interleukin 1 (IL 1) to support human B cell colony formation in semisolid cultures stimulated by staph protein A were analyzed. Human M phi subsets enriched for complement receptors (CR) effectively functioned as accessory cells supporting colony growth, whereas the responses obtained with CR-depleted M phi were 4.6-fold less. In experiments analyzing IL 1 production, CR-enriched M phi secreted four to 12 fold greater amounts of basal and stimulated IL 1 than CR-depleted M phi. Also, the addition of IL 1 to CR-depleted M phi resulted in a fourfold increase of colony numbers. The responses of cultures containing CR-depleted M phi plus IL 1, however, remained 30% less than those observed for cultures supplemented with CR-enriched M phi. Other studies showed that IL 1 was unable to substitute for M phi; the responses of cultures containing IL 1 and B cells were reduced 10-fold compared to cultures supplemented with autologous M phi. These findings indicate that human M phi subsets exist that differ in their ability to function as accessory cells. Although IL 1 can collaborate with certain M phi subsets to restore their accessory cell function, it cannot replace intact M phi. Thus, it is possible that other monokines or lymphokines play a role in M phi accessory cell function.
分析了人类外周血单核吞噬细胞（M phi）亚群以及白细胞介素1（IL 1）在葡萄球菌蛋白A刺激的半固体培养物中支持人类B细胞集落形成的能力。富含补体受体（CR）的人类M phi亚群有效地发挥辅助细胞的作用，支持集落生长，而用去除CR的M phi获得的反应则减少了4.6倍。在分析IL 1产生的实验中，富含CR的M phi分泌的基础和刺激的IL 1量比去除CR的M phi多4至12倍。此外，向去除CR的M phi中添加IL 1导致集落数量增加四倍。然而，含有去除CR的M phi加IL 1的培养物的反应仍比补充富含CR的M phi的培养物观察到的反应少30%。其他研究表明，IL 1不能替代M phi；与补充自体M phi的培养物相比，含有IL 1和B细胞的培养物的反应降低了10倍。这些发现表明，存在功能作为辅助细胞的能力不同的人类M phi亚群。虽然IL 1可以与某些M phi亚群协作以恢复其辅助细胞功能，但它不能替代完整的M phi。因此，其他单核因子或淋巴细胞因子可能在M phi辅助细胞功能中起作用。
