Rosenberg S A, Lipsky P E
J Immunol. 1980 Jul;125(1):232-7.
Supernatants from cultures of human mononuclear phagocytes (M phi) were found to support pokeweed mitogen- (PWM) induced generation of immunoglobulin-secreting cells (ISC) in cultures of T cell-depleted, human peripheral blood B cells. The M phi factor did not augment PWM-stimulated B cell proliferation. Moreover, M phi factor itself was not a polyclonal B cell activator, in that no ISC were generated unless PWM was present in the B cells cultures. M phi factor release, however, did not require PWM stimulation, but occurred spontaneously under standard conditions used to elicit the PWM response. The active factor found in M phi culture supernatants originated from M phi and not from the few contaminating T cells in the M phi population. M phi factor did not replace the requirement for T cells in this system, however, since factor activity was apparent only when B cell cultures contained a small number of T cells. Finally, M phi factor augmented generation of both IgM and IgG ISC. These data emphasize the important role of M phi in the differentiation of human B cells into ISC and indicate that at least some of the function of M phi in this system is accomplished by secreted factors.
在T细胞耗尽的人外周血B细胞培养物中，发现人单核吞噬细胞（M phi）培养物的上清液能够支持商陆丝裂原（PWM）诱导的免疫球蛋白分泌细胞（ISC）的生成。M phi因子不会增强PWM刺激的B细胞增殖。此外，M phi因子本身不是多克隆B细胞激活剂，因为除非B细胞培养物中存在PWM，否则不会产生ISC。然而，M phi因子的释放不需要PWM刺激，而是在用于引发PWM反应的标准条件下自发发生。在M phi培养上清液中发现的活性因子源自M phi，而非M phi群体中少量污染的T细胞。然而，M phi因子并不能替代该系统中对T细胞的需求，因为只有当B细胞培养物中含有少量T细胞时，因子活性才明显。最后，M phi因子增强了IgM和IgG ISC的生成。这些数据强调了M phi在人B细胞分化为ISC中的重要作用，并表明在该系统中M phi的至少一些功能是由分泌因子完成的。
