Bünger R, Sommer O, Walter G, Scholz R
Hoppe Seylers Z Physiol Chem. 1978 Jul;359(7):819-24. doi: 10.1515/bchm2.1978.359.2.819.
Effects of fructose and glucose on cardiac function and 14CO2 production from radioactive hexoses were studied in isolated working guinea pig hearts perfused at constant filling pressure via the left atrium. When hearts were perfused without exogenous substrates external work declined, indicating exhaustion of endogenous energy sources. It was possible to restore left ventricular function with concentrations of glucose in the physiological range; in order to achieve a similar restoration with fructose as the only exogenous substrate concentrations higher than 40mM were necessary. 14CO2 necessary. 14CO2 production from [U-14C]glucose was half-maximal at about 2mM glucose. On the other hand, 14CO2 production from [U-14C]fructose did not show saturation kinetics and, at concentrations around 2mM, was less than 5% of the rates observed with [U-14C]glucose. Moreover, fructose oxidation was suppressed in the presence of 5mM glucose. The data suggest that fructose cannot serve as a major substrate in the heart under in vivo conditions.
在通过左心房以恒定充盈压灌注的离体豚鼠工作心脏中，研究了果糖和葡萄糖对心脏功能以及放射性己糖产生14CO2的影响。当心脏在没有外源底物的情况下灌注时，外部功下降，表明内源性能量来源耗尽。用生理范围内的葡萄糖浓度可以恢复左心室功能；为了仅以果糖作为外源底物实现类似的恢复，需要高于40mM的浓度。从[U-14C]葡萄糖产生14CO2在约2mM葡萄糖时达到最大反应速度的一半。另一方面，从[U-14C]果糖产生14CO2未显示出饱和动力学，并且在约2mM的浓度下，其速率小于用[U-14C]葡萄糖观察到的速率的5%。此外，在5mM葡萄糖存在下，果糖氧化受到抑制。数据表明，在体内条件下，果糖不能作为心脏中的主要底物。
