Kettunen P
Scand J Clin Lab Invest. 1977 Dec;37(8):705-8. doi: 10.1080/00365517709101852.
Reactions of the cardiac muscle of the rat in vitro were investigated in the present study. Cardiac muscle was perfused by Langendorf's method; perfusion pressure was 80 cm H2O and the temperature of the perfusion solution 37 degrees C. The perfusion solution was aerated with a gas mixture of oxygen (95%) and carbon dioxide (5%). Potassium citrate solution was used for heart arrest, and heart function was recovered by infusion of Locke's solution, plus glucose, fructose or sucrose. During recovery the amplitude and frequenty of heart beats, the lactic acid in the drained perfusion solution, pH and potassium concentration were measured. The use of glucose, fructose or sucrose made no significant difference to any of these parameters. Next, the metabolism of glucose and fructose in the heart was investigated by means of D(U-14C)-glucose nad D(U-14C)-fructose. Radioactive lactic acid was detected in the drained perfusion solution with D(U-14C)-glucose, but not when D(U-14C)-fructose was used. The radioactivity incorporated into glycogen by the heart was also larger with D(U-14C)-glucose than with D(U-14C)-fructose. On a metabolic basis the use of glucose for resuscitation would seem to be more appropriate than fructose.
本研究对大鼠离体心肌的反应进行了调查。采用Langendorf法对心肌进行灌注；灌注压力为80厘米水柱，灌注液温度为37摄氏度。灌注液用氧气（95%）和二氧化碳（5%）的混合气体进行曝气。用柠檬酸钾溶液使心脏停搏，通过输注洛克溶液加葡萄糖、果糖或蔗糖来恢复心脏功能。在恢复过程中，测量心跳的幅度和频率、引流灌注液中的乳酸、pH值和钾浓度。使用葡萄糖、果糖或蔗糖对这些参数均无显著差异。接下来，通过D（U-14C）-葡萄糖和D（U-14C）-果糖研究了心脏中葡萄糖和果糖的代谢。用D（U-14C）-葡萄糖时，在引流的灌注液中检测到放射性乳酸，而使用D（U-14C）-果糖时则未检测到。心脏中糖原掺入的放射性也以D（U-14C）-葡萄糖比D（U-14C）-果糖时更高。从代谢角度来看，复苏时使用葡萄糖似乎比果糖更合适。
