Lee R M, McKenzie R, Kobayashi K, Garfield R E, Forrest J B, Daniel E E
J Microsc. 1982 Jan;125(Pt 1):77-88. doi: 10.1111/j.1365-2818.1982.tb00324.x.
The contribution of glutaraldehyde (GA) to the effective osmolarity of GA fixatives, the osmotic reactivity of the cells after fixation in GA, and also the duration of fixation in GA on cell volume, were investigated using cultured smooth muscle cells (SMC) and spiral aortic strips. Four fixation procedures were studied. We found that GA contributes to the total effective osmolarity of the fixatives, and that the type of buffers used for the fixatives can also affect the cell volume differently during GA fixation. After GA fixation, the cells were still osmotically reactive, regardless of the buffer types for making up the GA fixatives, so that the osmolarity of the wash buffer after GA fixation is important. However, OsO4 eliminates osmotic responses, thus the osmolarity of OsO4 fixative and wash buffer have negligible influence on the cell volume. Longer fixation time up to 4 h had no effect of the cell volume.
使用培养的平滑肌细胞（SMC）和主动脉螺旋条，研究了戊二醛（GA）对GA固定剂有效渗透压的贡献、GA固定后细胞的渗透反应性，以及GA固定时间对细胞体积的影响。研究了四种固定程序。我们发现GA对固定剂的总有效渗透压有贡献，并且用于固定剂的缓冲液类型在GA固定过程中也会对细胞体积产生不同影响。GA固定后，无论组成GA固定剂的缓冲液类型如何，细胞仍具有渗透反应性，因此GA固定后洗涤缓冲液的渗透压很重要。然而，OsO4消除了渗透反应，因此OsO4固定剂和洗涤缓冲液的渗透压对细胞体积的影响可忽略不计。长达4小时的较长固定时间对细胞体积没有影响。
