不同抗凝剂对红细胞谷胱甘肽过氧化物酶测定的影响
Effects of different anticoagulants on determination of erythrocyte glutathione peroxidase.
作者信息
Hussein K S, Jones B E
出版信息
Acta Vet Scand. 1981;22(3-4):472-9. doi: 10.1186/BF03548672.
Abstract
Glutathione peroxidase (GSH-Px) was determined in whole blood from cows, goats and horses using cumenehydroperoxide as substrate. Heparin was found to be the most suitable anticoagulant. The highest activities of GSH-Px were found with high concentrations of heparin in the blood samples (1000 and 1250 IU/ml of blood). Sodium fluoride and especially EDTA and sodium citrate gave lower activities of the enzyme. Storage of the blood samples at room temperature (20°C) or in a refrigerator (5°C) for 3 days resulted in significantly lower activities of the enzyme, especially in horse blood.
摘要
以氢过氧化异丙苯为底物，测定了奶牛、山羊和马全血中的谷胱甘肽过氧化物酶（GSH-Px）。发现肝素是最合适的抗凝剂。在血样中肝素浓度较高（1000和1250 IU/ml血液）时，GSH-Px的活性最高。氟化钠，尤其是乙二胺四乙酸（EDTA）和柠檬酸钠会使该酶的活性降低。血样在室温（约20°C）或冰箱（约5°C）中保存3天会导致该酶的活性显著降低，尤其是马血中的酶活性。
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引用本文的文献
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