牛和猪血液谷胱甘肽过氧化物酶测定方法
Procedure for blood glutathione peroxidase determination in cattle and swine.
作者信息
Agergaard N, Jensen P T
出版信息
Acta Vet Scand. 1982;23(4):515-27. doi: 10.1186/BF03546770.
Abstract
An improved testing system has been developed for direct measurement of glutathione peroxidase activity in heparinized whole blood at 37°C. Without loss in net yield the consumption of reagents has been found to be considerably lower with the new technique than with previously described techniques. Within the range 0–700 mKat/1 the GSH-Px activity in red cells may be measured with a high degree of accuracy and reproducibility without preceding separation and washing. The stability of the enzyme in bovine and porcine whole blood at 22°C, 4°C, and —20°C was determined.
摘要
已开发出一种改进的检测系统，用于在37°C下直接测量肝素化全血中谷胱甘肽过氧化物酶的活性。发现新技术在不损失净产量的情况下，试剂消耗量比先前描述的技术要低得多。在0-700 mKat/1范围内，无需预先分离和洗涤即可高精度和可重复地测量红细胞中的谷胱甘肽过氧化物酶（GSH-Px）活性。测定了该酶在牛和猪全血中在22°C、4°C和-20°C下的稳定性。
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