Green J A, Williams J, Levy H B
J Immunol. 1977 Jun;118(6):1936-43.
Mice lose demonstrable delayed hypersensitivity (DH) to DNFB, picryl chloride, or sheep red blood cells. Reconstitution of immune responsiveness can be accomplished by administration of cell-free lysates of spleens from mice with active DH to structurally related, but not to unrelated antigens. Peritoneal exudate cell lysates from mice with active DNFB-DH also restore DH to this antigen. Sera from sensitized mice, and sera and lymphoid tissue extracts from unsensitized mice are without activity. The restorative property of splenic lysates from DNFB-sensitized mice is unstable at 56 degrees C, not sedimented at 90,000 X G and inactivated by trypsin or magnesium ions. The presence of unexpressed, restorable DH may provide a biologic basis for the so called "transfer factor" phenomenon.
小鼠对二硝基氟苯（DNFB）、氯化苦基或绵羊红细胞失去明显的迟发型超敏反应（DH）。通过给具有活性DH的小鼠脾脏无细胞裂解物来重建免疫反应性，可使其对结构相关但非不相关的抗原产生反应。具有活性DNFB-DH的小鼠腹腔渗出细胞裂解物也能使对该抗原的DH得以恢复。致敏小鼠的血清以及未致敏小鼠的血清和淋巴组织提取物均无活性。DNFB致敏小鼠脾脏裂解物的恢复特性在56℃不稳定，在90,000×G下不沉淀，且会被胰蛋白酶或镁离子灭活。未表达但可恢复的DH的存在可能为所谓的“转移因子”现象提供生物学基础。
