Cataldo E, Reif A E
Cancer. 1982 Aug 1;50(3):531-42. doi: 10.1002/1097-0142(19820801)50:3<531::aid-cncr2820500325>3.0.co;2-u.
Pellets of 1 mg 9,10-dimethyl-1,3-benzanthracene (DMBA) were implanted into the submaxillary glands of 53 male C57BL/6J mice, and groups of mice were autopsied weekly or biweekly thereafter. Histologic evidence of tumor was noticed first at 12 weeks. From 16 weeks onwards, the submaxillary glands of all mice autopsied contained either carcinoma (three animals) or sarcoma (ten animals); 8/9 attempts to transplant these tumors in C57BL/6J mice were successful. Of the resulting eight tumor lines, two carcinomas and two fibrosarcomas were transplanted for over 30 months. Within the first few transplant generations, all four tumors showed an increase in growth rate and in histologic evidence of anaplasticity. For the particular tumors selected for study, the two carcinomas differed from the two sarcomas by growing more slowly, requiring more cells for tumor takes, and possessing a higher immunogenicity. These results may explain the types of tumors generated in submaxillary gland carcinogenesis. Carcinomas appear first, since they develop from the epithelial cells into which the carcinogen is implanted. Later, when fibroblasts wall off the carcinogen, fibroblasts are at risk for neoplastic conversion. Because of the more aggressive nature of the resultant fibrosarcoma cells, sarcomas may overgrow some early carcinomas.
将1毫克9,10 - 二甲基 - 1,3 - 苯并蒽（DMBA）丸剂植入53只雄性C57BL / 6J小鼠的颌下腺，此后每周或每两周对小鼠组进行尸检。在第12周首次发现肿瘤的组织学证据。从第16周起，所有接受尸检的小鼠颌下腺均含有癌（3只动物）或肉瘤（10只动物）；在C57BL / 6J小鼠中进行的这些肿瘤移植尝试，9次中有8次成功。在产生的8个肿瘤系中，2个癌和2个纤维肉瘤移植超过30个月。在最初的几代移植中，所有4种肿瘤的生长速度均加快，间变的组织学证据增多。对于所选用于研究的特定肿瘤，2种癌与2种肉瘤的不同之处在于生长较慢，肿瘤形成需要更多细胞，并且具有更高的免疫原性。这些结果可能解释颌下腺癌发生过程中产生的肿瘤类型。癌首先出现，因为它们由致癌物植入的上皮细胞发展而来。后来，当成纤维细胞将致癌物隔离时，成纤维细胞有发生肿瘤转化的风险。由于所得纤维肉瘤细胞的侵袭性更强，肉瘤可能会超过一些早期癌。
